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| | ==SOLUTION STRUCTURE OF THE CHITIN-BINDING DOMAIN OF BACILLUS CIRCULANS WL-12 CHITINASE A1== | | ==SOLUTION STRUCTURE OF THE CHITIN-BINDING DOMAIN OF BACILLUS CIRCULANS WL-12 CHITINASE A1== |
| - | <StructureSection load='1ed7' size='340' side='right'caption='[[1ed7]], [[NMR_Ensembles_of_Models | 30 NMR models]]' scene=''> | + | <StructureSection load='1ed7' size='340' side='right'caption='[[1ed7]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1ed7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_24 Atcc 24]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ED7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ED7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1ed7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Niallia_circulans Niallia circulans]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ED7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ED7 FirstGlance]. <br> |
| - | </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ed7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ed7 OCA], [http://pdbe.org/1ed7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ed7 RCSB], [http://www.ebi.ac.uk/pdbsum/1ed7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ed7 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ed7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ed7 OCA], [https://pdbe.org/1ed7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ed7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ed7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ed7 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/CHIA1_NIACI CHIA1_NIACI] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 24]] | |
| - | [[Category: Chitinase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Hashimoto, M]] | + | [[Category: Niallia circulans]] |
| - | [[Category: Ikegami, T]] | + | [[Category: Hashimoto M]] |
| - | [[Category: Okada, T]] | + | [[Category: Ikegami T]] |
| - | [[Category: Seino, S]] | + | [[Category: Okada T]] |
| - | [[Category: Shirakawa, M]] | + | [[Category: Seino S]] |
| - | [[Category: Watanabe, T]] | + | [[Category: Shirakawa M]] |
| - | [[Category: Hydrolase]]
| + | [[Category: Watanabe T]] |
| - | [[Category: Twisted beta-sandwich]]
| + | |
| Structural highlights
Function
CHIA1_NIACI
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The three-dimensional structure of the chitin-binding domain (ChBD) of chitinase A1 (ChiA1) from a Gram-positive bacterium, Bacillus circulans WL-12, was determined by means of multidimensional heteronuclear NMR methods. ChiA1 is a glycosidase that hydrolyzes chitin and is composed of an N-terminal catalytic domain, two fibronectin type III-like domains, and C-terminal ChBD(ChiA1) (45 residues, Ala(655)-Gln(699)), which binds specifically to insoluble chitin. ChBD(ChiA1) has a compact and globular structure with the topology of a twisted beta-sandwich. This domain contains two antiparallel beta-sheets, one composed of three strands and the other of two strands. The core region formed by the hydrophobic and aromatic residues makes the overall structure rigid and compact. The overall topology of ChBD(ChiA1) is similar to that of the cellulose-binding domain (CBD) of Erwinia chrysanthemi endoglucanase Z (CBD(EGZ)). However, ChBD(ChiA1) lacks the three aromatic residues aligned linearly and exposed to the solvent, which probably interact with cellulose in CBDs. Therefore, the binding mechanism of a group of ChBDs including ChBD(ChiA1) may be different from that proposed for CBDs.
Solution structure of the chitin-binding domain of Bacillus circulans WL-12 chitinase A1.,Ikegami T, Okada T, Hashimoto M, Seino S, Watanabe T, Shirakawa M J Biol Chem. 2000 May 5;275(18):13654-61. PMID:10788483[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Ikegami T, Okada T, Hashimoto M, Seino S, Watanabe T, Shirakawa M. Solution structure of the chitin-binding domain of Bacillus circulans WL-12 chitinase A1. J Biol Chem. 2000 May 5;275(18):13654-61. PMID:10788483
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