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| | ==RW16 peptide== | | ==RW16 peptide== |
| - | <StructureSection load='6rqs' size='340' side='right'caption='[[6rqs]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='6rqs' size='340' side='right'caption='[[6rqs]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6rqs]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RQS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RQS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6rqs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RQS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RQS FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=KFB:'>KFB</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rqs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rqs OCA], [http://pdbe.org/6rqs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rqs RCSB], [http://www.ebi.ac.uk/pdbsum/6rqs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rqs ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=KFB:5-Aminovaleric+Acid'>KFB</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rqs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rqs OCA], [https://pdbe.org/6rqs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rqs RCSB], [https://www.ebi.ac.uk/pdbsum/6rqs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rqs ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Alves, I]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Grelard, A]] | + | [[Category: Alves I]] |
| - | [[Category: Jobin, M L]] | + | [[Category: Grelard A]] |
| - | [[Category: Mackereth, C D]] | + | [[Category: Jobin M-L]] |
| - | [[Category: Arginine]]
| + | [[Category: Mackereth CD]] |
| - | [[Category: Cell-penetrating]]
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| - | [[Category: Membrane]]
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| - | [[Category: Membrane protein]]
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| - | [[Category: Tryptophan]]
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| Structural highlights
Publication Abstract from PubMed
Cell-penetrating peptides (CPPs) are short peptides that can translocate and transport cargoes into the intracellular milieu by crossing biological membranes. The mode of interaction and internalization of cell-penetrating peptides has long been controversial. While their interaction with anionic membranes is quite well understood, the insertion and behavior of CPPs in zwitterionic membranes, a major lipid component of eukaryotic cell membranes, is poorly studied. Herein, we investigated the membrane insertion of RW16 into zwitterionic membranes, a versatile CPP that also presents antibacterial and antitumor activities. Using complementary approaches, including NMR spectroscopy, fluorescence spectroscopy, circular dichroism, and molecular dynamic simulations, we determined the high-resolution structure of RW16 and measured its membrane insertion and orientation properties into zwitterionic membranes. Altogether, these results contribute to explaining the versatile properties of this peptide toward zwitterionic lipids.
Biophysical Insight on the Membrane Insertion of an Arginine-Rich Cell-Penetrating Peptide.,Jobin ML, Vamparys L, Deniau R, Grelard A, Mackereth CD, Fuchs PFJ, Alves ID Int J Mol Sci. 2019 Sep 9;20(18). pii: ijms20184441. doi: 10.3390/ijms20184441. PMID:31505894[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Jobin ML, Vamparys L, Deniau R, Grelard A, Mackereth CD, Fuchs PFJ, Alves ID. Biophysical Insight on the Membrane Insertion of an Arginine-Rich Cell-Penetrating Peptide. Int J Mol Sci. 2019 Sep 9;20(18). pii: ijms20184441. doi: 10.3390/ijms20184441. PMID:31505894 doi:http://dx.doi.org/10.3390/ijms20184441
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