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| | ==Cryo-EM structure of the Tc toxin TcdA1 in its pore state== | | ==Cryo-EM structure of the Tc toxin TcdA1 in its pore state== |
| - | <StructureSection load='5lki' size='340' side='right'caption='[[5lki]], [[Resolution|resolution]] 3.46Å' scene=''> | + | <SX load='5lki' size='340' side='right' viewer='molstar' caption='[[5lki]], [[Resolution|resolution]] 3.46Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5lki]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29999 Atcc 29999]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LKI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LKI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5lki]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Photorhabdus_luminescens Photorhabdus luminescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LKI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LKI FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5lkh|5lkh]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.46Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tcdA, tcdA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29488 ATCC 29999])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lki OCA], [https://pdbe.org/5lki PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lki RCSB], [https://www.ebi.ac.uk/pdbsum/5lki PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lki ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lki OCA], [http://pdbe.org/5lki PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lki RCSB], [http://www.ebi.ac.uk/pdbsum/5lki PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lki ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9RN43_PHOLU Q9RN43_PHOLU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| - | </StructureSection> | + | </SX> |
| - | [[Category: Atcc 29999]]
| + | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Gatsogiannis, C]] | + | [[Category: Photorhabdus luminescens]] |
| - | [[Category: Leidreiter, F]] | + | [[Category: Gatsogiannis C]] |
| - | [[Category: Merino, F]] | + | [[Category: Leidreiter F]] |
| - | [[Category: Meusch, D]] | + | [[Category: Merino F]] |
| - | [[Category: Prumbaum, D]] | + | [[Category: Meusch D]] |
| - | [[Category: Raunser, S]] | + | [[Category: Prumbaum D]] |
| - | [[Category: Roderer, D]] | + | [[Category: Raunser S]] |
| - | [[Category: Injection]]
| + | [[Category: Roderer D]] |
| - | [[Category: Nanodisc]]
| + | |
| - | [[Category: Pore-forming]]
| + | |
| - | [[Category: Toxin]]
| + | |
| Structural highlights
Function
Q9RN43_PHOLU
Publication Abstract from PubMed
Tc toxins from pathogenic bacteria use a special syringe-like mechanism to perforate the host cell membrane and inject a deadly enzyme into the host cytosol. The molecular mechanism of this unusual injection system is poorly understood. Using electron cryomicroscopy, we determined the structure of TcdA1 from Photorhabdus luminescens embedded in lipid nanodiscs. In our structure, compared with the previous structure of TcdA1 in the prepore state, the transmembrane helices rearrange in the membrane and open the initially closed pore. However, the helices do not span the complete membrane; instead, the loops connecting the helices form the rim of the funnel. Lipid head groups reach into the space between the loops and consequently stabilize the pore conformation. The linker domain is folded and packed into a pocket formed by the other domains of the toxin, thereby considerably contributing to stabilization of the pore state.
Membrane insertion of a Tc toxin in near-atomic detail.,Gatsogiannis C, Merino F, Prumbaum D, Roderer D, Leidreiter F, Meusch D, Raunser S Nat Struct Mol Biol. 2016 Aug 29. doi: 10.1038/nsmb.3281. PMID:27571177[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gatsogiannis C, Merino F, Prumbaum D, Roderer D, Leidreiter F, Meusch D, Raunser S. Membrane insertion of a Tc toxin in near-atomic detail. Nat Struct Mol Biol. 2016 Aug 29. doi: 10.1038/nsmb.3281. PMID:27571177 doi:http://dx.doi.org/10.1038/nsmb.3281
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