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| | <StructureSection load='6n5z' size='340' side='right'caption='[[6n5z]], [[Resolution|resolution]] 2.45Å' scene=''> | | <StructureSection load='6n5z' size='340' side='right'caption='[[6n5z]], [[Resolution|resolution]] 2.45Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6n5z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N5Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N5Z FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6n5z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N5Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6N5Z FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SEMA4C, KIAA1739, SEMAI, UNQ5855/PRO34487 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n5z OCA], [http://pdbe.org/6n5z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n5z RCSB], [http://www.ebi.ac.uk/pdbsum/6n5z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n5z ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6n5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n5z OCA], [https://pdbe.org/6n5z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6n5z RCSB], [https://www.ebi.ac.uk/pdbsum/6n5z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6n5z ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SNX5_HUMAN SNX5_HUMAN]] May be involved in several stages of intracellular trafficking. Plays a role in macropinocytosis. Plays a role in the internalization of EGFR after EGF stimulation.<ref>PMID:18854019</ref> <ref>PMID:21048941</ref> | + | [https://www.uniprot.org/uniprot/SNX5_HUMAN SNX5_HUMAN] May be involved in several stages of intracellular trafficking. Plays a role in macropinocytosis. Plays a role in the internalization of EGFR after EGF stimulation.<ref>PMID:18854019</ref> <ref>PMID:21048941</ref> [https://www.uniprot.org/uniprot/SEM4C_HUMAN SEM4C_HUMAN] Cell surface receptor for PLXNB2 that plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal brain development, axon guidance and cell migration (By similarity). Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade.<ref>PMID:17498836</ref> |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Protein trafficking requires coat complexes that couple recognition of sorting motifs in transmembrane cargoes with biogenesis of transport carriers. The mechanisms of cargo transport through the endosomal network are poorly understood. Here, we identify a sorting motif for endosomal recycling of cargoes, including the cation-independent mannose-6-phosphate receptor and semaphorin 4C, by the membrane tubulating BAR domain-containing sorting nexins SNX5 and SNX6. Crystal structures establish that this motif folds into a beta-hairpin, which binds a site in the SNX5/SNX6 phox homology domains. Over sixty cargoes share this motif and require SNX5/SNX6 for their recycling. These include cargoes involved in neuronal migration and a Drosophila snx6 mutant displays defects in axonal guidance. These studies identify a sorting motif and provide molecular insight into an evolutionary conserved coat complex, the 'Endosomal SNX-BAR sorting complex for promoting exit 1' (ESCPE-1), which couples sorting motif recognition to the BAR-domain-mediated biogenesis of cargo-enriched tubulo-vesicular transport carriers.
| + | |
| | | | |
| - | Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins.,Simonetti B, Paul B, Chaudhari K, Weeratunga S, Steinberg F, Gorla M, Heesom KJ, Bashaw GJ, Collins BM, Cullen PJ Nat Cell Biol. 2019 Oct;21(10):1219-1233. doi: 10.1038/s41556-019-0393-3. Epub, 2019 Oct 1. PMID:31576058<ref>PMID:31576058</ref>
| + | ==See Also== |
| - | | + | *[[Sorting nexin 3D structures|Sorting nexin 3D structures]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6n5z" style="background-color:#fffaf0;"></div>
| + | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Collins, B]] | + | [[Category: Collins B]] |
| - | [[Category: Paul, B]] | + | [[Category: Paul B]] |
| - | [[Category: Weeratunga, S]] | + | [[Category: Weeratunga S]] |
| - | [[Category: Endocytosis]]
| + | |
| - | [[Category: Endosome]]
| + | |
| - | [[Category: Snx]]
| + | |
| - | [[Category: Sorting nexin]]
| + | |
| Structural highlights
Function
SNX5_HUMAN May be involved in several stages of intracellular trafficking. Plays a role in macropinocytosis. Plays a role in the internalization of EGFR after EGF stimulation.[1] [2] SEM4C_HUMAN Cell surface receptor for PLXNB2 that plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal brain development, axon guidance and cell migration (By similarity). Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade.[3]
See Also
References
- ↑ Lim JP, Wang JT, Kerr MC, Teasdale RD, Gleeson PA. A role for SNX5 in the regulation of macropinocytosis. BMC Cell Biol. 2008 Oct 14;9:58. doi: 10.1186/1471-2121-9-58. PMID:18854019 doi:10.1186/1471-2121-9-58
- ↑ Wang JT, Kerr MC, Karunaratne S, Jeanes A, Yap AS, Teasdale RD. The SNX-PX-BAR family in macropinocytosis: the regulation of macropinosome formation by SNX-PX-BAR proteins. PLoS One. 2010 Oct 29;5(10):e13763. doi: 10.1371/journal.pone.0013763. PMID:21048941 doi:10.1371/journal.pone.0013763
- ↑ Wu H, Wang X, Liu S, Wu Y, Zhao T, Chen X, Zhu L, Wu Y, Ding X, Peng X, Yuan J, Wang X, Fan W, Fan M. Sema4C participates in myogenic differentiation in vivo and in vitro through the p38 MAPK pathway. Eur J Cell Biol. 2007 Jun;86(6):331-44. PMID:17498836 doi:10.1016/j.ejcb.2007.03.002
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