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3c98

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[[Image:3c98.jpg|left|200px]]
 
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==Revised structure of the munc18a-syntaxin1 complex==
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The line below this paragraph, containing "STRUCTURE_3c98", creates the "Structure Box" on the page.
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<StructureSection load='3c98' size='340' side='right'caption='[[3c98]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3c98]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1dn1 1dn1]. The November 2013 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''SNARE Proteins'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2013_11 10.2210/rcsb_pdb/mom_2013_11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C98 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3C98 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.601&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3c98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c98 OCA], [https://pdbe.org/3c98 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3c98 RCSB], [https://www.ebi.ac.uk/pdbsum/3c98 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3c98 ProSAT]</span></td></tr>
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{{STRUCTURE_3c98| PDB=3c98 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/STXB1_RAT STXB1_RAT] May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c9/3c98_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3c98 ConSurf].
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<div style="clear:both"></div>
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'''Revised structure of the munc18a-syntaxin1 complex'''
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==See Also==
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*[[Syntaxin 3D structures|Syntaxin 3D structures]]
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*[[Syntaxin-binding protein|Syntaxin-binding protein]]
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==Overview==
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__TOC__
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Sec1/Munc18-like (SM) proteins functionally interact with SNARE proteins in vesicular fusion. Despite their high sequence conservation, structurally disparate binding modes for SM proteins with syntaxins have been observed. Several SM proteins appear to bind only to a short peptide present at the N terminus of syntaxin, designated the N-peptide, while Munc18a binds to a 'closed' conformation formed by the remaining portion of syntaxin 1a. Here, we show that the syntaxin 16 N-peptide binds to the SM protein Vps45, but the remainder of syntaxin 16 strongly enhances the affinity of the interaction. Likewise, the N-peptide of syntaxin 1a serves as a second binding site in the Munc18a/syntaxin 1a complex. When the syntaxin 1a N-peptide is bound to Munc18a, SNARE complex formation is blocked. Removal of the N-peptide enables binding of syntaxin 1a to its partner SNARE SNAP-25, while still bound to Munc18a. This suggests that Munc18a controls the accessibility of syntaxin 1a to its partners, a role that might be common to all SM proteins.
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</StructureSection>
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[[Category: Large Structures]]
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==About this Structure==
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[[Category: RCSB PDB Molecule of the Month]]
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3C98 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1dn1 1dn1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C98 OCA].
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==Reference==
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Munc18a controls SNARE assembly through its interaction with the syntaxin N-peptide., Burkhardt P, Hattendorf DA, Weis WI, Fasshauer D, EMBO J. 2008 Mar 13;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18337752 18337752]
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[[Category: Protein complex]]
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[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Burkhardt, P.]]
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[[Category: SNARE Proteins]]
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[[Category: Fasshauer, D.]]
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[[Category: Burkhardt P]]
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[[Category: Hattendorf, D A.]]
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[[Category: Fasshauer D]]
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[[Category: Misura, K M.]]
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[[Category: Hattendorf DA]]
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[[Category: Scheller, R H.]]
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[[Category: Misura KM]]
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[[Category: Weis, W I.]]
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[[Category: Scheller RH]]
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[[Category: Alternative splicing]]
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[[Category: Weis WI]]
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[[Category: Coiled coil]]
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[[Category: Cytoplasm]]
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[[Category: Endocytosis/exocytosis complex]]
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[[Category: Membrane]]
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[[Category: Neurotransmitter transport]]
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[[Category: Phosphoprotein]]
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[[Category: Protein complex]]
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[[Category: Protein transport]]
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[[Category: Transmembrane]]
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[[Category: Transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 30 13:39:25 2008''
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Current revision

Revised structure of the munc18a-syntaxin1 complex

PDB ID 3c98

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