1fs7

From Proteopedia

(Difference between revisions)

Current revision

CYTOCHROME C NITRITE REDUCTASE FROM WOLINELLA SUCCINOGENES

Structural highlights

1fs7 is a 1 chain structure with sequence from Wolinella succinogenes. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.6Å
Ligands:	, , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NRFA_WOLSU Plays a role in nitrite reduction.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Cytochrome c nitrite reductase catalyzes the 6-electron reduction of nitrite to ammonia. This second part of the respiratory pathway of nitrate ammonification is a key step in the biological nitrogen cycle. The x-ray structure of the enzyme from the epsilon-proteobacterium Wolinella succinogenes has been solved to a resolution of 1.6 A. It is a pentaheme c-type cytochrome whose heme groups are packed in characteristic motifs that also occur in other multiheme cytochromes. Structures of W. succinogenes nitrite reductase have been obtained with water bound to the active site heme iron as well as complexes with two inhibitors, sulfate and azide, whose binding modes and inhibitory functions differ significantly. Cytochrome c nitrite reductase is part of a highly optimized respiratory system found in a wide range of Gram-negative bacteria. It reduces both anionic and neutral substrates at the distal side of a lysine-coordinated high-spin heme group, which is accessible through two different channels, allowing for a guided flow of reaction educt and product. Based on sequence comparison and secondary structure prediction, we have demonstrated that cytochrome c nitrite reductases constitute a protein family of high structural similarity.

Cytochrome c nitrite reductase from Wolinella succinogenes. Structure at 1.6 A resolution, inhibitor binding, and heme-packing motifs.,Einsle O, Stach P, Messerschmidt A, Simon J, Kroger A, Huber R, Kroneck PM J Biol Chem. 2000 Dec 15;275(50):39608-16. PMID:10984487^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Simon J, Gross R, Einsle O, Kroneck PM, Kroger A, Klimmek O. A NapC/NirT-type cytochrome c (NrfH) is the mediator between the quinone pool and the cytochrome c nitrite reductase of Wolinella succinogenes. Mol Microbiol. 2000 Feb;35(3):686-96. PMID:10672190
↑ Einsle O, Stach P, Messerschmidt A, Simon J, Kroger A, Huber R, Kroneck PM. Cytochrome c nitrite reductase from Wolinella succinogenes. Structure at 1.6 A resolution, inhibitor binding, and heme-packing motifs. J Biol Chem. 2000 Dec 15;275(50):39608-16. PMID:10984487 doi:10.1074/jbc.M006188200

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1fs7"

@@ Line 3: / Line 3: @@
 <StructureSection load='1fs7' size='340' side='right'caption='[[1fs7]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1fs7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Wolinella_succinogenes Wolinella succinogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FS7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FS7 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1fs7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Wolinella_succinogenes Wolinella succinogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FS7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FS7 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=Y1:YTTRIUM+ION'>Y1</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1fs8|1fs8]], [[1fs9|1fs9]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=Y1:YTTRIUM+ION'>Y1</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fs7 OCA], [http://pdbe.org/1fs7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fs7 RCSB], [http://www.ebi.ac.uk/pdbsum/1fs7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1fs7 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fs7 OCA], [https://pdbe.org/1fs7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fs7 RCSB], [https://www.ebi.ac.uk/pdbsum/1fs7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fs7 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/NRFA_WOLSU NRFA_WOLSU]] Plays a role in nitrite reduction.<ref>PMID:10672190</ref>
+[https://www.uniprot.org/uniprot/NRFA_WOLSU NRFA_WOLSU] Plays a role in nitrite reduction.<ref>PMID:10672190</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 15: / Line 15: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fs/1fs7_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 32: / Line 32: @@
 ==See Also==
 *[[Cytochrome C 3D structures|Cytochrome C 3D structures]]
-*[[Nitrite reductase|Nitrite reductase]]
+*[[Cytochrome c nitrite reductase|Cytochrome c nitrite reductase]]
 == References ==
 <references/>
@@ Line 39: / Line 39: @@
 [[Category: Large Structures]]
 [[Category: Wolinella succinogenes]]
-[[Category: Einsle, O]]
+[[Category: Einsle O]]
-[[Category: Huber, R]]
+[[Category: Huber R]]
-[[Category: Kroeger, A]]
+[[Category: Kroeger A]]
-[[Category: Kroneck, P M.H]]
+[[Category: Kroneck PMH]]
-[[Category: Messerschmidt, A]]
+[[Category: Messerschmidt A]]
-[[Category: Simon, J]]
+[[Category: Simon J]]
-[[Category: Stach, P]]
+[[Category: Stach P]]
-[[Category: C-type cytochrome]]
-[[Category: Oxidoreductase]]

1fs7

From Proteopedia

Current revision

CYTOCHROME C NITRITE REDUCTASE FROM WOLINELLA SUCCINOGENES

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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