1ej0

From Proteopedia

(Difference between revisions)

Current revision

FTSJ RNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYLMETHIONINE, MERCURY DERIVATIVE

Structural highlights

1ej0 is a 1 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.5Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RLME_ECOLI Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Structural, biochemical, and genetic techniques were applied to investigate the function of FtsJ, a recently identified heat shock protein. FtsJ is well conserved, from bacteria to humans. The 1.5 A crystal structure of FtsJ in complex with its cofactor S-adenosylmethionine revealed that FtsJ has a methyltransferase fold. The molecular surface of FtsJ exposes a putative nucleic acid binding groove composed of highly conserved, positively charged residues. Substrate analysis showed that FtsJ methylates 23S rRNA within 50S ribosomal subunits in vitro and in vivo. Null mutations in ftsJ show a dramatically altered ribosome profile, a severe growth disadvantage, and a temperature-sensitive phenotype. Our results reveal an unexpected link between the heat shock response and RNA metabolism.

RNA methylation under heat shock control.,Bugl H, Fauman EB, Staker BL, Zheng F, Kushner SR, Saper MA, Bardwell JC, Jakob U Mol Cell. 2000 Aug;6(2):349-60. PMID:10983982^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Caldas T, Binet E, Bouloc P, Costa A, Desgres J, Richarme G. The FtsJ/RrmJ heat shock protein of Escherichia coli is a 23 S ribosomal RNA methyltransferase. J Biol Chem. 2000 Jun 2;275(22):16414-9. PMID:10748051 doi:http://dx.doi.org/10.1074/jbc.M001854200
↑ Bugl H, Fauman EB, Staker BL, Zheng F, Kushner SR, Saper MA, Bardwell JC, Jakob U. RNA methylation under heat shock control. Mol Cell. 2000 Aug;6(2):349-60. PMID:10983982

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ej0"

@@ Line 3: / Line 3: @@
 <StructureSection load='1ej0' size='340' side='right'caption='[[1ej0]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1ej0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EJ0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EJ0 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1ej0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EJ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EJ0 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1eiz|1eiz]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ej0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ej0 OCA], [http://pdbe.org/1ej0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ej0 RCSB], [http://www.ebi.ac.uk/pdbsum/1ej0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ej0 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ej0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ej0 OCA], [https://pdbe.org/1ej0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ej0 RCSB], [https://www.ebi.ac.uk/pdbsum/1ej0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ej0 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/RLME_ECOLI RLME_ECOLI]] Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit.<ref>PMID:10748051</ref>
+[https://www.uniprot.org/uniprot/RLME_ECOLI RLME_ECOLI] Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit.<ref>PMID:10748051</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 33: / Line 33: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillus coli migula 1895]]
+[[Category: Escherichia coli]]
 [[Category: Large Structures]]
-[[Category: Bardwell, J C.A]]
+[[Category: Bardwell JCA]]
-[[Category: Bugl, H]]
+[[Category: Bugl H]]
-[[Category: Fauman, E B]]
+[[Category: Fauman EB]]
-[[Category: Jakob, U]]
+[[Category: Jakob U]]
-[[Category: Kushner, S R]]
+[[Category: Kushner SR]]
-[[Category: Saper, M A]]
+[[Category: Saper MA]]
-[[Category: Staker, B L]]
+[[Category: Staker BL]]
-[[Category: Zheng, F]]
+[[Category: Zheng F]]
-[[Category: 23s ribosomal rna]]
-[[Category: Adenosyl methionine]]
-[[Category: Adomet]]
-[[Category: Ftsj]]
-[[Category: Heat shock protein]]
-[[Category: Methyltransferase]]
-[[Category: Transferase]]

1ej0

From Proteopedia

Current revision

FTSJ RNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYLMETHIONINE, MERCURY DERIVATIVE

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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