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| | ==XNF7 BBOX, DEVELOPMENTAL PROTEIN, PH 7.5, 30 C, WITH ZINC, NMR, 1 STRUCTURE== | | ==XNF7 BBOX, DEVELOPMENTAL PROTEIN, PH 7.5, 30 C, WITH ZINC, NMR, 1 STRUCTURE== |
| - | <StructureSection load='1fre' size='340' side='right'caption='[[1fre]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''> | + | <StructureSection load='1fre' size='340' side='right'caption='[[1fre]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1fre]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/African_clawed_frog African clawed frog]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FRE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FRE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1fre]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FRE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FRE FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fre FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fre OCA], [http://pdbe.org/1fre PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fre RCSB], [http://www.ebi.ac.uk/pdbsum/1fre PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1fre ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fre FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fre OCA], [https://pdbe.org/1fre PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fre RCSB], [https://www.ebi.ac.uk/pdbsum/1fre PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fre ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/NF7B_XENLA NF7B_XENLA]] Transcription factor that determines dorsal-ventral body axis.<ref>PMID:1936552</ref> | + | [https://www.uniprot.org/uniprot/NF7B_XENLA NF7B_XENLA] Transcription factor that determines dorsal-ventral body axis.<ref>PMID:1936552</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: African clawed frog]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Borden, K L.B]] | + | [[Category: Xenopus laevis]] |
| - | [[Category: Freemont, P S]] | + | [[Category: Borden KLB]] |
| - | [[Category: Bbox]] | + | [[Category: Freemont PS]] |
| - | [[Category: Development]]
| + | |
| - | [[Category: Mid-blastula-transition]]
| + | |
| - | [[Category: Xnf7]]
| + | |
| - | [[Category: Zinc-binding protein]]
| + | |
| Structural highlights
Function
NF7B_XENLA Transcription factor that determines dorsal-ventral body axis.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Xenopus nuclear factor XNF7, a maternally expressed protein, functions in patterning of the embryo. XNF7 contains a number of defined protein domains implicated in the regulation of some developmental processes. Among these is a tripartite motif comprising a zinc-binding RING finger and B-box domain next to a predicted alpha-helical coiled-coil domain. Interestingly, this motif is found in a variety of protein including several proto-oncoproteins. Here we describe the solution structure of the XNF7 B-box zinc-binding domain determined at physiological pH by 1H NMR methods. The B-box structure represents the first three-dimensional structure of this new motif and comprises a monomer have two beta-strands, two helical turns and three extended loop regions packed in a novel topology. The r.m.s. deviation for the best 18 structures is 1.15 A for backbone atoms and 1.94 A for all atoms. Structure calculations and biochemical data shows one zinc atom ligated in a Cys2-His2 tetrahedral arrangement. We have used mutant peptides to determine the metal ligation scheme which surprisingly shows that not all of the seven conserved cysteines/histidines in the B-box motif are involved in metal ligation. The B-box structure is not similar in tertiary fold to any other known zinc-binding motif.
Novel topology of a zinc-binding domain from a protein involved in regulating early Xenopus development.,Borden KL, Lally JM, Martin SR, O'Reilly NJ, Etkin LD, Freemont PS EMBO J. 1995 Dec 1;14(23):5947-56. PMID:8846787[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Reddy BA, Kloc M, Etkin L. The cloning and characterization of a maternally expressed novel zinc finger nuclear phosphoprotein (xnf7) in Xenopus laevis. Dev Biol. 1991 Nov;148(1):107-16. PMID:1936552
- ↑ Borden KL, Lally JM, Martin SR, O'Reilly NJ, Etkin LD, Freemont PS. Novel topology of a zinc-binding domain from a protein involved in regulating early Xenopus development. EMBO J. 1995 Dec 1;14(23):5947-56. PMID:8846787
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