6r5w
From Proteopedia
(Difference between revisions)
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<StructureSection load='6r5w' size='340' side='right'caption='[[6r5w]], [[Resolution|resolution]] 1.70Å' scene=''> | <StructureSection load='6r5w' size='340' side='right'caption='[[6r5w]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6r5w]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R5W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6R5W FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6r5w]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_psa Bacteriophage psa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R5W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6R5W FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r5w OCA], [http://pdbe.org/6r5w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r5w RCSB], [http://www.ebi.ac.uk/pdbsum/6r5w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r5w ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r5w OCA], [http://pdbe.org/6r5w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r5w RCSB], [http://www.ebi.ac.uk/pdbsum/6r5w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r5w ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bacteriophages provide excellent tools for diagnostics, remediation, and targeted microbiome manipulation, yet isolating viruses with suitable host specificity remains challenging. Using Listeria phage PSA, we present a synthetic biology blueprint for host-range engineering through targeted modification of serovar-specific receptor binding proteins (RBPs). We identify Gp15 as the PSA RBP and construct a synthetic phage library featuring sequence-randomized RBPs, from which host range mutants are isolated and subsequently integrated into a synthetic, polyvalent phage with extended host range. To enable rational design of chimeric RBPs, we determine the crystal structure of the Gp15 receptor-binding carboxyl terminus at 1.7-A resolution and employ bioinformatics to identify compatible, prophage-encoded RBPs targeting different Listeria serovars. Structure-guided design enables exchange of heterologous RBP head, neck, or shoulder domains to generate chimeric phages with predictable and extended host ranges. These strategies will facilitate the development of phage biologics based on standardized virus scaffolds with tunable host specificities. | ||
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| + | Reprogramming Bacteriophage Host Range through Structure-Guided Design of Chimeric Receptor Binding Proteins.,Dunne M, Rupf B, Tala M, Qabrati X, Ernst P, Shen Y, Sumrall E, Heeb L, Pluckthun A, Loessner MJ, Kilcher S Cell Rep. 2019 Oct 29;29(5):1336-1350.e4. doi: 10.1016/j.celrep.2019.09.062. PMID:31665644<ref>PMID:31665644</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6r5w" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Bacteriophage psa]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Dunne, M]] | [[Category: Dunne, M]] | ||
Current revision
Crystal structure of the receptor binding protein (gp15) of Listeria phage PSA
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