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1ht9

From Proteopedia

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DOMAIN SWAPPING EF-HANDS

Structural highlights

1ht9 is a 2 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.76Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

S100G_BOVIN

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The structure of calbindin D(9k) with two substitutions was determined by X-ray crystallography at 1.8-A resolution. Unlike wild-type calbindin D(9k), which is a monomeric protein with two EF-hands, the structure of the mutated calbindin D(9k) reveals an intertwined dimer. In the dimer, two EF-hands of the monomers have exchanged places, and thus a 3D domain-swapped dimer has been formed. EF-hand I of molecule A is packed toward EF-hand II of molecule B and vice versa. The formation of a hydrophobic cluster, in a region linking the EF-hands, promotes the conversion of monomers to 3D domain-swapped dimers. We propose a mechanism by which domain swapping takes place via the apo form of calbindin D(9k). Once formed, the calbindin D(9k) dimers are remarkably stable, as with even larger misfolded aggregates like amyloids. Thus calbindin D(9k) dimers cannot be converted to monomers by dilution. However, heating can be used for conversion, indicating high energy barriers separating monomers from dimers.

An extended hydrophobic core induces EF-hand swapping.,Hakansson M, Svensson A, Fast J, Linse S Protein Sci. 2001 May;10(5):927-33. PMID:11316872^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hakansson M, Svensson A, Fast J, Linse S. An extended hydrophobic core induces EF-hand swapping. Protein Sci. 2001 May;10(5):927-33. PMID:11316872 doi:10.1110/ps.47501

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ht9"

@@ Line 3: / Line 3: @@
 <StructureSection load='1ht9' size='340' side='right'caption='[[1ht9]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1ht9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HT9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HT9 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1ht9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HT9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HT9 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.76&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ht9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ht9 OCA], [http://pdbe.org/1ht9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ht9 RCSB], [http://www.ebi.ac.uk/pdbsum/1ht9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ht9 ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ht9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ht9 OCA], [https://pdbe.org/1ht9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ht9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ht9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ht9 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/S100G_BOVIN S100G_BOVIN]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 28: / Line 31: @@
 ==See Also==
-*[[S100 protein|S100 protein]]
+*[[S100 proteins 3D structures|S100 proteins 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Bovin]]
+[[Category: Bos taurus]]
 [[Category: Large Structures]]
-[[Category: Fast, J]]
+[[Category: Fast J]]
-[[Category: Hakansson, M]]
+[[Category: Hakansson M]]
-[[Category: Linse, S]]
+[[Category: Linse S]]
-[[Category: Svensson, A L]]
+[[Category: Svensson AL]]
-[[Category: Calbindin d9k]]
-[[Category: Calcium binding]]
-[[Category: Domain swapping]]
-[[Category: Ef-hand]]
-[[Category: Folding]]
-[[Category: Metal binding protein]]

1ht9

From Proteopedia

Current revision

DOMAIN SWAPPING EF-HANDS

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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