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| | ==STRUCTURE OF A PARALYTIC PEPTIDE FROM AN INSECT, MANDUCA SEXTA== | | ==STRUCTURE OF A PARALYTIC PEPTIDE FROM AN INSECT, MANDUCA SEXTA== |
| - | <StructureSection load='1hrl' size='340' side='right'caption='[[1hrl]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='1hrl' size='340' side='right'caption='[[1hrl]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1hrl]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HRL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HRL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1hrl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Manduca_sexta Manduca sexta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HRL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HRL FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hrl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hrl OCA], [http://pdbe.org/1hrl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1hrl RCSB], [http://www.ebi.ac.uk/pdbsum/1hrl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1hrl ProSAT]</span></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hrl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hrl OCA], [https://pdbe.org/1hrl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hrl RCSB], [https://www.ebi.ac.uk/pdbsum/1hrl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hrl ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PAP1_MANSE PAP1_MANSE]] Causes rapid, rigid paralysis when injected into Lepidopteran larvae. The physiological role may be to reduce hemolymph loss following injury and promote wound healing. | + | [https://www.uniprot.org/uniprot/PAP1_MANSE PAP1_MANSE] Causes rapid, rigid paralysis when injected into Lepidopteran larvae. The physiological role may be to reduce hemolymph loss following injury and promote wound healing. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Kanost, M R]] | + | [[Category: Manduca sexta]] |
| - | [[Category: Prakash, O]] | + | [[Category: Kanost MR]] |
| - | [[Category: Yu, X Q]] | + | [[Category: Prakash O]] |
| - | [[Category: Enf family]] | + | [[Category: Yu X-Q]] |
| - | [[Category: Paralytic peptide]]
| + | |
| - | [[Category: Plasmatocyte spreading peptide]]
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| - | [[Category: Toxin]]
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| Structural highlights
Function
PAP1_MANSE Causes rapid, rigid paralysis when injected into Lepidopteran larvae. The physiological role may be to reduce hemolymph loss following injury and promote wound healing.
Publication Abstract from PubMed
Paralytic peptide 1 (PP1) from a moth, Manduca sexta, is a 23-residue peptide (Glu-Asn-Phe-Ala-Gly-Gly-Cys-Ala-Thr-Gly-Tyr-Leu-Arg-Thr-Ala-Asp-Gly-Arg -Cys-Lys-Pro-Thr-Phe) that was first found to have paralytic activity when injected into M. sexta larvae. Recent studies demonstrated that PP1 also stimulated the spreading and aggregation of a blood cell type called plasmatocytes and inhibited bleeding from wounds. We determined the solution structure of PP1 by two-dimensional 1H NMR spectroscopy to begin to understand structural-functional relationships of this peptide. PP1 has an ordered structure, which is composed of a short antiparallel beta-sheet at residues Tyr11-Thr14 and Arg18-Pro21, three beta turns at residues Phe3-Gly6, Ala8-Tyr11 and Thr14-Gly17, and a half turn at the carboxyl-terminus (residues Lys20-Phe23). The well-defined secondary and tertiary structure was stabilized by hydrogen bonding and side-chain hydrophobic interactions. In comparison with two related insect peptides, whose structures have been solved recently, the amino-terminal region of PP1 is substantially more ordered. The short antiparallel beta-sheet of PP1 has a folding pattern similar to the carboxyl-terminal subdomain of epidermal growth factor (EGF). Therefore, PP1 may interact with EGF receptor-like molecules to trigger its different biological activities.
Structure of a paralytic peptide from an insect, Manduca sexta.,Yu XQ, Prakash O, Kanost MR J Pept Res. 1999 Sep;54(3):256-61. PMID:10517164[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Yu XQ, Prakash O, Kanost MR. Structure of a paralytic peptide from an insect, Manduca sexta. J Pept Res. 1999 Sep;54(3):256-61. PMID:10517164
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