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| | ==Three dimensional solution structure of huwentoxin-II by 2D 1H-NMR== | | ==Three dimensional solution structure of huwentoxin-II by 2D 1H-NMR== |
| - | <StructureSection load='1i25' size='340' side='right'caption='[[1i25]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='1i25' size='340' side='right'caption='[[1i25]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1i25]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ornithoctonus_huwena Ornithoctonus huwena]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I25 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I25 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1i25]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cyriopagopus_schmidti Cyriopagopus schmidti]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I25 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I25 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i25 OCA], [http://pdbe.org/1i25 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1i25 RCSB], [http://www.ebi.ac.uk/pdbsum/1i25 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1i25 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i25 OCA], [https://pdbe.org/1i25 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i25 RCSB], [https://www.ebi.ac.uk/pdbsum/1i25 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i25 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TXH21_HAPSC TXH21_HAPSC]] Lethal neurotoxin that blocks neuromuscular transmission. Acts cooperatively to potentiate the activity of huwentoxin-I. This toxin is active against insects.<ref>PMID:10424342</ref> | + | [https://www.uniprot.org/uniprot/TXH21_CYRSC TXH21_CYRSC] Lethal neurotoxin that blocks neuromuscular transmission. Acts cooperatively to potentiate the activity of huwentoxin-I. This toxin is active against insects.<ref>PMID:10424342</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 1i25" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 1i25" style="background-color:#fffaf0;"></div> |
| - | | |
| - | ==See Also== | |
| - | *[[NMR Ensembles of Models|NMR Ensembles of Models]] | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Cyriopagopus schmidti]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ornithoctonus huwena]]
| + | [[Category: Gu XC]] |
| - | [[Category: Gu, X C]] | + | [[Category: Liang SP]] |
| - | [[Category: Liang, S P]] | + | [[Category: Lu SY]] |
| - | [[Category: Lu, S Y]] | + | [[Category: Shu Q]] |
| - | [[Category: Shu, Q]] | + | |
| - | [[Category: Disulfide bond]]
| + | |
| - | [[Category: Insecticidal toxin]]
| + | |
| - | [[Category: Neurotoxin]]
| + | |
| - | [[Category: Toxin]]
| + | |
| Structural highlights
Function
TXH21_CYRSC Lethal neurotoxin that blocks neuromuscular transmission. Acts cooperatively to potentiate the activity of huwentoxin-I. This toxin is active against insects.[1]
Publication Abstract from PubMed
The three-dimensional structure of huwentoxin-II (HWTX-II), an insecticidal peptide purified from the venom of spider Selenocosmia huwena with a unique disulfide bond linkage as I-III, II-V, and IV-VI, has been determined using 2D (1)H-NMR. The resulting structure of HWTX-II contains two beta-turns (C4-S7 and K24-W27) and a double-stranded antiparallel beta-sheet (W27-C29 and C34-K36). Although the C-terminal double-stranded beta-sheet cross-linked by two disulfide bonds (II-V and IV-VI in HWTX-II, II-V and III-VI in the ICK molecules) is conserved both in HWTX-II and the ICK molecules, the structure of HWTX-II is unexpected absence of the cystine knot because of its unique disulfide linkage. It suggests that HWTX-II adopts a novel scaffold different from the ICK motif that is adopted by all other spider toxin structures elucidated thus far. Furthermore, the structure of HWTX-II, which conforms to the disulfide-directed beta-hairpin (DDH) motif, not only supports the hypothesis that the ICK is a minor elaboration of the more ancestral DDH motif but also suggests that HWTX-II may have evolved from the same structural ancestor.
The structure of spider toxin huwentoxin-II with unique disulfide linkage: evidence for structural evolution.,Shu Q, Lu SY, Gu XC, Liang SP Protein Sci. 2002 Feb;11(2):245-52. PMID:11790834[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Shu Q, Liang SP. Purification and characterization of huwentoxin-II, a neurotoxic peptide from the venom of the Chinese bird spider Selenocosmia huwena. J Pept Res. 1999 May;53(5):486-91. PMID:10424342
- ↑ Shu Q, Lu SY, Gu XC, Liang SP. The structure of spider toxin huwentoxin-II with unique disulfide linkage: evidence for structural evolution. Protein Sci. 2002 Feb;11(2):245-52. PMID:11790834
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