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| | ==STRUCTURE OF INTERLEUKIN 16: IMPLICATIONS FOR FUNCTION, NMR, 20 STRUCTURES== | | ==STRUCTURE OF INTERLEUKIN 16: IMPLICATIONS FOR FUNCTION, NMR, 20 STRUCTURES== |
| - | <StructureSection load='1i16' size='340' side='right'caption='[[1i16]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='1i16' size='340' side='right'caption='[[1i16]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1i16]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I16 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I16 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1i16]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I16 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I16 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i16 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i16 OCA], [http://pdbe.org/1i16 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1i16 RCSB], [http://www.ebi.ac.uk/pdbsum/1i16 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1i16 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i16 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i16 OCA], [https://pdbe.org/1i16 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i16 RCSB], [https://www.ebi.ac.uk/pdbsum/1i16 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i16 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/IL16_HUMAN IL16_HUMAN]] Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.<ref>PMID:14734747</ref> <ref>PMID:18097041</ref> Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.<ref>PMID:14734747</ref> <ref>PMID:18097041</ref> Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.<ref>PMID:14734747</ref> <ref>PMID:18097041</ref> | + | [https://www.uniprot.org/uniprot/IL16_HUMAN IL16_HUMAN] Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.<ref>PMID:14734747</ref> <ref>PMID:18097041</ref> Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.<ref>PMID:14734747</ref> <ref>PMID:18097041</ref> Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.<ref>PMID:14734747</ref> <ref>PMID:18097041</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ambrosius, D]] | + | [[Category: Ambrosius D]] |
| - | [[Category: Baier, M]] | + | [[Category: Baier M]] |
| - | [[Category: Ciosto, C]] | + | [[Category: Ciosto C]] |
| - | [[Category: Georgescu, J]] | + | [[Category: Georgescu J]] |
| - | [[Category: Holak, T A]] | + | [[Category: Holak TA]] |
| - | [[Category: Kurth, R]] | + | [[Category: Kurth R]] |
| - | [[Category: Lang, K]] | + | [[Category: Lang K]] |
| - | [[Category: Lanzendoerfer, M]] | + | [[Category: Lanzendoerfer M]] |
| - | [[Category: Muehlhahn, P]] | + | [[Category: Muehlhahn P]] |
| - | [[Category: Renner, C]] | + | [[Category: Renner C]] |
| - | [[Category: Zweckstetter, M]] | + | [[Category: Zweckstetter M]] |
| - | [[Category: Cytokine]]
| + | |
| - | [[Category: Lymphocyte chemoattractant factor]]
| + | |
| - | [[Category: Pdz domain]]
| + | |
| Structural highlights
Function
IL16_HUMAN Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.[1] [2] Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.[3] [4] Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.[5] [6]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The structure of a folded core of IL-16 is similar to that of intracellular protein modules called PDZ domains. IL-16 is thus the first extracellular protein found to have a PDZ-like fold. However, it does not exhibit normal peptide binding properties of PDZ domains. This is due to alterations of the structure at the 'PDZ-like binding site' of IL-16 (the GLGF cleft): the GLGF cleft of IL-16 is much smaller than those of PDZ-domains and is additionally blocked with a tryptophan side chain at its center. Our experiments indicate also that IL-16 nonspecifically aggregates in solution; but formation of a homo-tetrameric protein is not required, in contrast to previous suggestions, for its chemo-attractant activity.
Structure of interleukin 16 resembles a PDZ domain with an occluded peptide binding site.,Muhlhahn P, Zweckstetter M, Georgescu J, Ciosto C, Renner C, Lanzendorfer M, Lang K, Ambrosius D, Baier M, Kurth R, Holak TA Nat Struct Biol. 1998 Aug;5(8):682-6. PMID:9699630[7]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Center DM, Cruikshank WW, Zhang Y. Nuclear pro-IL-16 regulation of T cell proliferation: p27(KIP1)-dependent G0/G1 arrest mediated by inhibition of Skp2 transcription. J Immunol. 2004 Feb 1;172(3):1654-60. PMID:14734747
- ↑ Zhang Y, Tuzova M, Xiao ZX, Cruikshank WW, Center DM. Pro-IL-16 recruits histone deacetylase 3 to the Skp2 core promoter through interaction with transcription factor GABP. J Immunol. 2008 Jan 1;180(1):402-8. PMID:18097041
- ↑ Center DM, Cruikshank WW, Zhang Y. Nuclear pro-IL-16 regulation of T cell proliferation: p27(KIP1)-dependent G0/G1 arrest mediated by inhibition of Skp2 transcription. J Immunol. 2004 Feb 1;172(3):1654-60. PMID:14734747
- ↑ Zhang Y, Tuzova M, Xiao ZX, Cruikshank WW, Center DM. Pro-IL-16 recruits histone deacetylase 3 to the Skp2 core promoter through interaction with transcription factor GABP. J Immunol. 2008 Jan 1;180(1):402-8. PMID:18097041
- ↑ Center DM, Cruikshank WW, Zhang Y. Nuclear pro-IL-16 regulation of T cell proliferation: p27(KIP1)-dependent G0/G1 arrest mediated by inhibition of Skp2 transcription. J Immunol. 2004 Feb 1;172(3):1654-60. PMID:14734747
- ↑ Zhang Y, Tuzova M, Xiao ZX, Cruikshank WW, Center DM. Pro-IL-16 recruits histone deacetylase 3 to the Skp2 core promoter through interaction with transcription factor GABP. J Immunol. 2008 Jan 1;180(1):402-8. PMID:18097041
- ↑ Muhlhahn P, Zweckstetter M, Georgescu J, Ciosto C, Renner C, Lanzendorfer M, Lang K, Ambrosius D, Baier M, Kurth R, Holak TA. Structure of interleukin 16 resembles a PDZ domain with an occluded peptide binding site. Nat Struct Biol. 1998 Aug;5(8):682-6. PMID:9699630 doi:10.1038/1376
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