6l6v
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6l6v is ON HOLD Authors: Liu, B., Wang, Z. Description: SPO1 Gp44 N-terminal region (1-55) Category: Unreleased Structures Category: Liu, B...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==SPO1 Gp44 N-terminal region (1-55)== | |
| + | <StructureSection load='6l6v' size='340' side='right'caption='[[6l6v]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6l6v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_virus_SPO1 Bacillus virus SPO1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L6V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L6V FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l6v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l6v OCA], [https://pdbe.org/6l6v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l6v RCSB], [https://www.ebi.ac.uk/pdbsum/6l6v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l6v ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/GP44_BPSP1 GP44_BPSP1] Hypothesized to function in the shutoff of host biosyntheses. But it seems dispensable both for host shutoff and for phage multiplication. Its shutoff function is probably not entirely specific to host activities. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | DNA mimicry by proteins is a strategy that employed by some proteins to occupy the binding sites of the DNA-binding proteins and deny further access to these sites by DNA. Such proteins have been found in bacteriophage, eukaryotic virus, prokaryotic, and eukaryotic cells to imitate non-coding functions of DNA. Here, we report another phage protein Gp44 from bacteriophage SPO1 of Bacillus subtilis, employing mimicry as part of unusual strategy to inhibit host RNA polymerase. Consisting of three simple domains, Gp44 contains a DNA binding motif, a flexible DNA mimic domain and a random-coiled domain. Gp44 is able to anchor to host genome and interact bacterial RNA polymerase via the beta and beta' subunit, resulting in bacterial growth inhibition. Our findings represent a non-specific strategy that SPO1 phage uses to target different bacterial transcription machinery regardless of the structural variations of RNA polymerases. This feature may have potential applications like generation of genetic engineered phages with Gp44 gene incorporated used in phage therapy to target a range of bacterial hosts. | ||
| - | + | A Bacteriophage DNA Mimic Protein Employs a Non-specific Strategy to Inhibit the Bacterial RNA Polymerase.,Wang Z, Wang H, Mulvenna N, Sanz-Hernandez M, Zhang P, Li Y, Ma J, Wang Y, Matthews S, Wigneshweraraj S, Liu B Front Microbiol. 2021 Jun 2;12:692512. doi: 10.3389/fmicb.2021.692512., eCollection 2021. PMID:34149677<ref>PMID:34149677</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Liu | + | <div class="pdbe-citations 6l6v" style="background-color:#fffaf0;"></div> |
| - | [[Category: Wang | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bacillus virus SPO1]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Liu B]] | ||
| + | [[Category: Wang Z]] | ||
Current revision
SPO1 Gp44 N-terminal region (1-55)
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