6uug
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of methanesulfinate monooxygenase MsuC from Pseudomonas fluorescens at 1.69 angstrom resolution== | |
| + | <StructureSection load='6uug' size='340' side='right'caption='[[6uug]], [[Resolution|resolution]] 1.69Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6uug]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens_Pf0-1 Pseudomonas fluorescens Pf0-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UUG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6UUG FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.685Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6uug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uug OCA], [https://pdbe.org/6uug PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6uug RCSB], [https://www.ebi.ac.uk/pdbsum/6uug PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6uug ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q3K9A0_PSEPF Q3K9A0_PSEPF] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Methyl sulfur compounds are a rich source of environmental sulfur for microorganisms, but their use requires redox systems. The bacterial sfn and msu operons contain two-component flavin-dependent monooxygenases for dimethylsulfone (DMSO2) assimilation: SfnG converts DMSO2 to methanesulfinate (MSI(-)), and MsuD converts methanesulfonate (MS(-)) to sulfite. However, the enzymatic oxidation of MSI(-) to MS(-) has not been demonstrated, and the function of the last enzyme of the msu operon (MsuC) is unresolved. We employed crystallographic and biochemical studies to identify the function of MsuC from Pseudomonas fluorescens. The crystal structure of MsuC adopts the acyl-CoA dehydrogenase fold with putative binding sites for flavin and MSI(-), and functional assays of MsuC in the presence of its oxidoreductase MsuE, FMN, and NADH confirm the enzymatic generation of MS(-). These studies reveal that MsuC converts MSI(-) to MS(-) in sulfite biosynthesis from DMSO2. | ||
| - | + | Structure and function of the two-component flavin-dependent methanesulfinate monooxygenase within bacterial sulfur assimilation.,Soule J, Gnann AD, Gonzalez R, Parker MJ, McKenna KC, Nguyen SV, Phan NT, Wicht DK, Dowling DP Biochem Biophys Res Commun. 2019 Nov 18. pii: S0006-291X(19)32128-X. doi:, 10.1016/j.bbrc.2019.11.008. PMID:31753487<ref>PMID:31753487</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6uug" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pseudomonas fluorescens Pf0-1]] | ||
| + | [[Category: Dowling DP]] | ||
| + | [[Category: Gnann AD]] | ||
| + | [[Category: Gonzalez R]] | ||
| + | [[Category: McKenna KC]] | ||
| + | [[Category: Nguyen SV]] | ||
| + | [[Category: Parker MJ]] | ||
| + | [[Category: Phan NT]] | ||
| + | [[Category: Soule J]] | ||
| + | [[Category: Wicht DK]] | ||
Current revision
Structure of methanesulfinate monooxygenase MsuC from Pseudomonas fluorescens at 1.69 angstrom resolution
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