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| | <StructureSection load='1jeh' size='340' side='right'caption='[[1jeh]], [[Resolution|resolution]] 2.40Å' scene=''> | | <StructureSection load='1jeh' size='340' side='right'caption='[[1jeh]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1jeh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JEH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JEH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1jeh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JEH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JEH FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydrolipoyl_dehydrogenase Dihydrolipoyl dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.4 1.8.1.4] </span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jeh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jeh OCA], [http://pdbe.org/1jeh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jeh RCSB], [http://www.ebi.ac.uk/pdbsum/1jeh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1jeh ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jeh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jeh OCA], [https://pdbe.org/1jeh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jeh RCSB], [https://www.ebi.ac.uk/pdbsum/1jeh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jeh ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/DLDH_YEAST DLDH_YEAST]] Lipoamide dehydrogenase is a component of the alpha-ketoacid dehydrogenase complexes. This includes the pyruvate dehydrogenase complex, which catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). Acts also as component of the glycine cleavage system (glycine decarboxylase complex), which catalyzes the degradation of glycine. | + | [https://www.uniprot.org/uniprot/DLDH_YEAST DLDH_YEAST] Lipoamide dehydrogenase is a component of the alpha-ketoacid dehydrogenase complexes. This includes the pyruvate dehydrogenase complex, which catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). Acts also as component of the glycine cleavage system (glycine decarboxylase complex), which catalyzes the degradation of glycine. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Dihydrolipoyl dehydrogenase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Saccharomyces cerevisiae]] | | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Reed, J]] | + | [[Category: Reed J]] |
| - | [[Category: Sekigushi, T]] | + | [[Category: Sekigushi T]] |
| - | [[Category: Suzuki, K]] | + | [[Category: Suzuki K]] |
| - | [[Category: Takenaka, A]] | + | [[Category: Takenaka A]] |
| - | [[Category: Toyoda, T]] | + | [[Category: Toyoda T]] |
| - | [[Category: 2-oxoglutarate dehydrogenase complex]]
| + | |
| - | [[Category: Oxidoreductase]]
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| - | [[Category: Pyruvate dehydrogenase complex]]
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| Structural highlights
Function
DLDH_YEAST Lipoamide dehydrogenase is a component of the alpha-ketoacid dehydrogenase complexes. This includes the pyruvate dehydrogenase complex, which catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). Acts also as component of the glycine cleavage system (glycine decarboxylase complex), which catalyzes the degradation of glycine.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The crystal structure of eucaryotic lipoamide dehydrogenase from yeast has been determined by an X-ray analysis at 2.7 (partially at 2.4) A resolution. The enzyme has two identical subunits related by a pseudo twofold symmetry. The tertiary structure is similar to those of other procaryotic enzymes. The active site, consisting of FAD, Cys44, and Cys49 from one subunit and His457' from the other subunit, is highly conserved. This enzyme is directly bound to the core protein E2 of the 2-oxoglutarate dehydrogenase complex, whereas it is bound to the pyruvate dehydrogenase complex through a protein X. The calculated electrostatic potential suggests two characteristic regions for binding with these two proteins.
Crystal structure of eucaryotic E3, lipoamide dehydrogenase from yeast.,Toyoda T, Suzuki K, Sekiguchi T, Reed LJ, Takenaka A J Biochem. 1998 Apr;123(4):668-74. PMID:9538259[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Toyoda T, Suzuki K, Sekiguchi T, Reed LJ, Takenaka A. Crystal structure of eucaryotic E3, lipoamide dehydrogenase from yeast. J Biochem. 1998 Apr;123(4):668-74. PMID:9538259
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