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| | ==Structural transitions as determinants of calcium-dependent antibiotic daptomycin== | | ==Structural transitions as determinants of calcium-dependent antibiotic daptomycin== |
| - | <StructureSection load='1t5n' size='340' side='right'caption='[[1t5n]], [[NMR_Ensembles_of_Models | 17 NMR models]]' scene=''> | + | <StructureSection load='1t5n' size='340' side='right'caption='[[1t5n]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1t5n]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T5N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T5N FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1t5n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_filamentosus Streptomyces filamentosus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T5N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T5N FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DKA:DECANOIC+ACID'>DKA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 17 models</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=DSG:D-ASPARAGINE'>DSG</scene>, <scene name='pdbligand=DSN:D-SERINE'>DSN</scene>, <scene name='pdbligand=KYN:(2S)-2-AMINO-4-(2-AMINOPHENYL)-4-OXOBUTANOIC+ACID'>KYN</scene>, <scene name='pdbligand=LME:(2S,3R)-2-AZANYL-3-METHYL-PENTANEDIOIC+ACID'>LME</scene>, <scene name='pdbligand=ORN:L-ORNITHINE'>ORN</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=DKA:DECANOIC+ACID'>DKA</scene>, <scene name='pdbligand=DSG:D-ASPARAGINE'>DSG</scene>, <scene name='pdbligand=DSN:D-SERINE'>DSN</scene>, <scene name='pdbligand=KYN:(2S)-2-AMINO-4-(2-AMINOPHENYL)-4-OXOBUTANOIC+ACID'>KYN</scene>, <scene name='pdbligand=LME:(2S,3R)-2-AZANYL-3-METHYL-PENTANEDIOIC+ACID'>LME</scene>, <scene name='pdbligand=ORN:L-ORNITHINE'>ORN</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1xt7|1xt7]], [[1t5m|1t5m]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t5n OCA], [https://pdbe.org/1t5n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t5n RCSB], [https://www.ebi.ac.uk/pdbsum/1t5n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t5n ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t5n OCA], [http://pdbe.org/1t5n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t5n RCSB], [http://www.ebi.ac.uk/pdbsum/1t5n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1t5n ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Hancock, R E]] | + | [[Category: Streptomyces filamentosus]] |
| - | [[Category: Jung, D]] | + | [[Category: Hancock RE]] |
| - | [[Category: Okon, M]] | + | [[Category: Jung D]] |
| - | [[Category: Rozek, A]] | + | [[Category: Okon M]] |
| - | [[Category: Antibiotic]]
| + | [[Category: Rozek A]] |
| - | [[Category: Calcium-dependent]]
| + | |
| - | [[Category: Cubicin]]
| + | |
| - | [[Category: Daptomycin]]
| + | |
| - | [[Category: Lipopeptide]]
| + | |
| Structural highlights
1t5n is a 1 chain structure with sequence from Streptomyces filamentosus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | Solution NMR, 17 models |
| Ligands: | , , , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Publication Abstract from PubMed
Daptomycin is a cyclic anionic lipopeptide antibiotic recently approved for the treatment of complicated skin infections (Cubicin). Its function is dependent on calcium (as Ca2+). Circular dichroism spectroscopy indicated that daptomycin experienced two structural transitions: a transition upon interaction of daptomycin with Ca2+, and a further transition upon interaction with Ca2+ and the bacterial acidic phospholipid, phosphatidyl glycerol. The Ca2+-dependent insertion of daptomycin into model membranes promoted mild and more pronounced perturbations as assessed by the increase of lipid flip-flop and membrane leakage, respectively. The NMR structure of daptomycin indicated that Ca2+ induced a conformational change in daptomycin that increased its amphipathicity. These results are consistent with the hypothesis that the association of Ca2+ with daptomycin permits it to interact with bacterial membranes with effects that are similar to those of the cationic antimicrobial peptides.
Structural transitions as determinants of the action of the calcium-dependent antibiotic daptomycin.,Jung D, Rozek A, Okon M, Hancock RE Chem Biol. 2004 Jul;11(7):949-57. PMID:15271353[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Jung D, Rozek A, Okon M, Hancock RE. Structural transitions as determinants of the action of the calcium-dependent antibiotic daptomycin. Chem Biol. 2004 Jul;11(7):949-57. PMID:15271353 doi:10.1016/j.chembiol.2004.04.020
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