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| | <StructureSection load='5vkf' size='340' side='right'caption='[[5vkf]], [[Resolution|resolution]] 2.75Å' scene=''> | | <StructureSection load='5vkf' size='340' side='right'caption='[[5vkf]], [[Resolution|resolution]] 2.75Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5vkf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43588 Atcc 43588]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VKF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VKF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5vkf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylothermus_marinus Staphylothermus marinus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VKF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VKF FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NPY:NAPHTHALENE'>NPY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.752Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5vh0|5vh0]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NPY:NAPHTHALENE'>NPY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vkf OCA], [http://pdbe.org/5vkf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vkf RCSB], [http://www.ebi.ac.uk/pdbsum/5vkf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vkf ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vkf OCA], [https://pdbe.org/5vkf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vkf RCSB], [https://www.ebi.ac.uk/pdbsum/5vkf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vkf ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q54436_STAMA Q54436_STAMA] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 43588]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: McDougall, M]] | + | [[Category: Staphylothermus marinus]] |
| - | [[Category: Stetefeld, J]] | + | [[Category: McDougall M]] |
| - | [[Category: Nanotube]] | + | [[Category: Stetefeld J]] |
| - | [[Category: Passive sample device]]
| + | |
| - | [[Category: Polycyclic aromatic hydrocarbon]]
| + | |
| - | [[Category: Surface layer]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
Q54436_STAMA
Publication Abstract from PubMed
Polycyclic aromatic hydrocarbons (PAHs) are toxic, mutagenic and among the most damaging chemical compounds with regard to living organisms. Because of their persistence and wide distribution removal from the environment is an important challenge. Here we report a new Nano container matrix based on the deep sea archaea-derived RHCC-Nanotube (RHCC-NT), which rapidly and preferentially binds low molecular weight PAHs. Under controlled-laboratory conditions and using fluorescence spectroscopy in combination with X-ray crystallography and MD simulations, we quantified the real-time binding of low molecular weight PAHs (2-4 rings) to our substrate. Binding coefficients ranged from 5.4 +/- 1.6 (fluorene) to 32 +/- 7.0 muM (acenaphthylene) and a binding capacity of 85 pmoles PAH per mg RHCC-NT, or 2.12 mumoles in a standard 25 mg sampler. The uptake rate of pyrene was calculated to be 1.59 nmol/hrmol RHCC-NT (at 10 C). Our results clearly show that RHCC-NT is uniquely suited as a monitoring matrix for low molecular weight PAHs.
Proteinaceous Nano container Encapsulate Polycyclic Aromatic Hydrocarbons.,McDougall M, Francisco O, Harder-Viddal C, Roshko R, Heide F, Sidhu S, Khajehpour M, Leslie J, Palace V, Tomy GT, Stetefeld J Sci Rep. 2019 Jan 31;9(1):1058. doi: 10.1038/s41598-018-37323-x. PMID:30705306[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ McDougall M, Francisco O, Harder-Viddal C, Roshko R, Heide F, Sidhu S, Khajehpour M, Leslie J, Palace V, Tomy GT, Stetefeld J. Proteinaceous Nano container Encapsulate Polycyclic Aromatic Hydrocarbons. Sci Rep. 2019 Jan 31;9(1):1058. doi: 10.1038/s41598-018-37323-x. PMID:30705306 doi:http://dx.doi.org/10.1038/s41598-018-37323-x
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