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| | ==Cryo-EM reconstruction of Sulfolobus polyhedral virus 1 (SPV1)== | | ==Cryo-EM reconstruction of Sulfolobus polyhedral virus 1 (SPV1)== |
| - | <StructureSection load='6oj0' size='340' side='right'caption='[[6oj0]], [[Resolution|resolution]] 3.70Å' scene=''> | + | <SX load='6oj0' size='340' side='right' viewer='molstar' caption='[[6oj0]], [[Resolution|resolution]] 3.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6oj0]] is a 43 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_polyhedral_virus_1 Sulfolobus polyhedral virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OJ0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OJ0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6oj0]] is a 43 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_polyhedral_virus_1 Sulfolobus polyhedral virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OJ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OJ0 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6oj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oj0 OCA], [http://pdbe.org/6oj0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oj0 RCSB], [http://www.ebi.ac.uk/pdbsum/6oj0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oj0 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.7Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6oj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oj0 OCA], [https://pdbe.org/6oj0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6oj0 RCSB], [https://www.ebi.ac.uk/pdbsum/6oj0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6oj0 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/CAPS1_SPV1 CAPS1_SPV1] VP4 self-assembles to form, together with capsid protein VP10, an icosahedral caspid of 87 nm in diameter, with a T=43 symmetry and composed of 420 hexamers and 12 pentamers (PubMed:31636205). VP4 proteins arrange into hexons, while VP10 proteins form the pentameric densities located at the 5-fold axes in the virion (PubMed:31636205). The stoichiometry of VP4:VP10 is 42:1 (PubMed:31636205).<ref>PMID:31636205</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| - | </StructureSection> | + | </SX> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Sulfolobus polyhedral virus 1]] | | [[Category: Sulfolobus polyhedral virus 1]] |
| - | [[Category: Conway, J F]] | + | [[Category: Conway JF]] |
| - | [[Category: Egelman, E H]] | + | [[Category: Egelman EH]] |
| - | [[Category: Krupovic, M]] | + | [[Category: Krupovic M]] |
| - | [[Category: Liu, Y]] | + | [[Category: Liu Y]] |
| - | [[Category: Prangishvili, D]] | + | [[Category: Prangishvili D]] |
| - | [[Category: Wang, F]] | + | [[Category: Wang F]] |
| - | [[Category: Archaeal pilus]]
| + | |
| - | [[Category: Icosahedral symmetry]]
| + | |
| - | [[Category: Structural protein]]
| + | |
| - | [[Category: Virus]]
| + | |
| Structural highlights
Function
CAPS1_SPV1 VP4 self-assembles to form, together with capsid protein VP10, an icosahedral caspid of 87 nm in diameter, with a T=43 symmetry and composed of 420 hexamers and 12 pentamers (PubMed:31636205). VP4 proteins arrange into hexons, while VP10 proteins form the pentameric densities located at the 5-fold axes in the virion (PubMed:31636205). The stoichiometry of VP4:VP10 is 42:1 (PubMed:31636205).[1]
Publication Abstract from PubMed
Studies on viruses infecting archaea living in the most extreme environments continue to show a remarkable diversity of structures, suggesting that the sampling continues to be very sparse. We have used electron cryo-microscopy to study at 3.7-A resolution the structure of the Sulfolobus polyhedral virus 1 (SPV1), which was originally isolated from a hot, acidic spring in Beppu, Japan. The 2 capsid proteins with variant single jelly-roll folds form pentamers and hexamers which assemble into a T = 43 icosahedral shell. In contrast to tailed icosahedral double-stranded DNA (dsDNA) viruses infecting bacteria and archaea, and herpesviruses infecting animals and humans, where naked DNA is packed under very high pressure due to the repulsion between adjacent layers of DNA, the circular dsDNA in SPV1 is fully covered with a viral protein forming a nucleoprotein filament with attractive interactions between layers. Most strikingly, we have been able to show that the DNA is in an A-form, as it is in the filamentous viruses infecting hyperthermophilic acidophiles. Previous studies have suggested that DNA is in the B-form in bacteriophages, and our study is a direct visualization of the structure of DNA in an icosahedral virus.
A packing for A-form DNA in an icosahedral virus.,Wang F, Liu Y, Su Z, Osinski T, de Oliveira GAP, Conway JF, Schouten S, Krupovic M, Prangishvili D, Egelman EH Proc Natl Acad Sci U S A. 2019 Oct 21. pii: 1908242116. doi:, 10.1073/pnas.1908242116. PMID:31636205[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wang F, Liu Y, Su Z, Osinski T, de Oliveira GAP, Conway JF, Schouten S, Krupovic M, Prangishvili D, Egelman EH. A packing for A-form DNA in an icosahedral virus. Proc Natl Acad Sci U S A. 2019 Oct 21. pii: 1908242116. doi:, 10.1073/pnas.1908242116. PMID:31636205 doi:http://dx.doi.org/10.1073/pnas.1908242116
- ↑ Wang F, Liu Y, Su Z, Osinski T, de Oliveira GAP, Conway JF, Schouten S, Krupovic M, Prangishvili D, Egelman EH. A packing for A-form DNA in an icosahedral virus. Proc Natl Acad Sci U S A. 2019 Oct 21. pii: 1908242116. doi:, 10.1073/pnas.1908242116. PMID:31636205 doi:http://dx.doi.org/10.1073/pnas.1908242116
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