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6r1i

From Proteopedia

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Current revision

Structure of porcine Aichi virus polymerase

Structural highlights

6r1i is a 2 chain structure with sequence from Porcine kobuvirus swine/S-1-HUN/2007/Hungary. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.634Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

B8R1T8_9PICO

Publication Abstract from PubMed

RNA-dependent RNA polymerase 3D(pol) is a key enzyme for the replication of picornaviruses. The viral genome is translated into a single polyprotein that is subsequently proteolytically processed into matured products. The 3D(pol) enzyme arises from a stable 3CD precursor that has high proteolytic activity but no polymerase activity. Upon cleavage of the precursor the newly established N-terminus of 3D(pol) is liberated and inserts itself into a pocket on the surface of the 3D(pol) enzyme. The essential residue for this mechanism is the very first glycine that is conserved among almost all picornaviruses. However, kobuviruses and siciniviruses have a serine residue instead. Intrigued by this anomaly we sought to solve the crystal structure of these 3D(pol) enzymes. The structures revealed a unique fold of the 3D(pol) N-termini but the very first serine residues were inserted into a charged pocket in a similar manner as the glycine residue in other picornaviruses. These structures revealed a common underlying mechanism of 3D(pol) activation that lies in activation of the alpha10 helix containing a key catalytical residue Asp238 that forms a hydrogen bond with the 2' hydroxyl group of the incoming NTP nucleotide.

Structures of kobuviral and siciniviral polymerases reveal conserved mechanism of picornaviral polymerase activation.,Dubankova A, Horova V, Klima M, Boura E J Struct Biol. 2019 Aug 12. pii: S1047-8477(19)30174-1. doi:, 10.1016/j.jsb.2019.08.004. PMID:31415898^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dubankova A, Horova V, Klima M, Boura E. Structures of kobuviral and siciniviral polymerases reveal conserved mechanism of picornaviral polymerase activation. J Struct Biol. 2019 Aug 12. pii: S1047-8477(19)30174-1. doi:, 10.1016/j.jsb.2019.08.004. PMID:31415898 doi:http://dx.doi.org/10.1016/j.jsb.2019.08.004

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6r1i"

Categories: Large Structures | Porcine kobuvirus swine/S-1-HUN/2007/Hungary | Boura E | Dubankova A

@@ Line 3: / Line 3: @@
 <StructureSection load='6r1i' size='340' side='right'caption='[[6r1i]], [[Resolution|resolution]] 2.63&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6r1i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Kobuvirus_swine/s-1-hun/2007/hungary Kobuvirus swine/s-1-hun/2007/hungary]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R1I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6R1I FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6r1i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Porcine_kobuvirus_swine/S-1-HUN/2007/Hungary Porcine kobuvirus swine/S-1-HUN/2007/Hungary]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R1I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6R1I FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r1i OCA], [http://pdbe.org/6r1i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r1i RCSB], [http://www.ebi.ac.uk/pdbsum/6r1i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r1i ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.634&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6r1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r1i OCA], [https://pdbe.org/6r1i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6r1i RCSB], [https://www.ebi.ac.uk/pdbsum/6r1i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6r1i ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/B8R1T8_9PICO B8R1T8_9PICO]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Kobuvirus swine/s-1-hun/2007/hungary]]
 [[Category: Large Structures]]
-[[Category: Boura, E]]
+[[Category: Porcine kobuvirus swine/S-1-HUN/2007/Hungary]]
-[[Category: Dubankova, A]]
+[[Category: Boura E]]
-[[Category: Polymerase]]
+[[Category: Dubankova A]]
-[[Category: Rna]]
-[[Category: Transferase]]
-[[Category: Virus]]

6r1i

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Current revision

Structure of porcine Aichi virus polymerase

Structural highlights

Function

Publication Abstract from PubMed

References

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