1jgu

<table><tr><td colspan='2'>[[1jgu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JGU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JGU FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HBC:(2-AMINO-3-PHENYL-BICYCLO[2.2.1]HEPT-2-YL)-PHENYL-METHANONE'>HBC</scene>, <scene name='pdbligand=OH:HYDROXIDE+ION'>OH</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1jgv|1jgv]]</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jgu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jgu OCA], [http://pdbe.org/1jgu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jgu RCSB], [http://www.ebi.ac.uk/pdbsum/1jgu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1jgu ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Murine antibody 1D4 selectively catalyzes a highly disfavored beta-elimination reaction. Crystal structures of unliganded 1D4 and 1D4 in complex with a transition-state analog (TSA) have elucidated a possible general base mode of catalysis. The structures of the unliganded and liganded Fabs were determined to 1.80 and 1.85 A resolution, respectively. The structure of the complex reveals a binding pocket with high shape complementarity to the TSA, which is recruited to coerce the substrate into the sterically demanding, eclipsed conformation that is required for catalysis. A histidine residue and two water molecules are likely involved in the catalysis. The structure supports either a concerted E2 or stepwise E1cB-like mechanism for elimination. Finally, the liganded 1D4 structure shows minor conformational rearrangements in CDR H2, indicative of induced-fit binding of the hapten. 1D4 has pushed the boundaries of antibody-mediated catalysis into the realm of disfavored reactions and, hence, represents an important milestone in the development of this technology.

Structural basis for a disfavored elimination reaction in catalytic antibody 1D4.,Larsen NA, Heine A, Crane L, Cravatt BF, Lerner RA, Wilson IA J Mol Biol. 2001 Nov 16;314(1):93-102. PMID:11724535<ref>PMID:11724535</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 1jgu" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Crane, L]]

[[Category: Cravatt, B F]]

[[Category: Heine, A]]

[[Category: Larsen, N A]]

[[Category: Lerner, R A]]

[[Category: Wilson, I A]]

[[Category: Immune system]]