This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

1jjx

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

Solution Structure of Recombinant Human Brain-type Fatty acid Binding Protein

Structural highlights

1jjx is a 1 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

FABP7_HUMAN B-FABP could be involved in the transport of a so far unknown hydrophobic ligand with potential morphogenic activity during CNS development. It is required for the establishment of the radial glial fiber system in developing brain, a system that is necessary for the migration of immature neurons to establish cortical layers (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Human brain-type fatty acid-binding protein (B-FABP) has been recombinantly expressed in Escherichia coli both unlabelled and 15N-enriched for structure investigation in solution using high-resolution NMR spectroscopy. The sequential assignments of the 1H and 15N resonances were achieved by applying multidimensional homo- and heteronuclear NMR experiments. The ensemble of the 20 final energy-minimized structures, representing human B-FABP in solution, have been calculated based on a total of 2490 meaningful distance constraints. The overall B-FABP structure exhibits the typical backbone conformation described for other members of the FABP family, consisting often antiparallel beta-strands (betaA to betaJ) that form two almost orthogonal beta-sheets, a helix-turn-helix motif that closes the beta-barrel on one side, and a short N-terminal helical loop. A comparison with the crystal structure of the same protein complexed with docosahexaenoic acid reveals only minor differences in both secondary structure and overall topology. Moreover, the NMR data indicate a close structural relationship between human B-FABP and heart-type FABP with respect to fatty acid binding inside the protein cavity.

Solution structure of fatty acid-binding protein from human brain.,Rademacher M, Zimmerman AW, Ruterjans H, Veerkamp JH, Lucke C Mol Cell Biochem. 2002 Oct;239(1-2):61-8. PMID:12479569^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Rademacher M, Zimmerman AW, Ruterjans H, Veerkamp JH, Lucke C. Solution structure of fatty acid-binding protein from human brain. Mol Cell Biochem. 2002 Oct;239(1-2):61-8. PMID:12479569

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1jjx"

@@ Line 1: / Line 1: @@
 ==Solution Structure of Recombinant Human Brain-type Fatty acid Binding Protein==
-<StructureSection load='1jjx' size='340' side='right'caption='[[1jjx]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='1jjx' size='340' side='right'caption='[[1jjx]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1jjx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JJX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JJX FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1jjx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JJX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JJX FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1fdq|1fdq]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jjx OCA], [http://pdbe.org/1jjx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jjx RCSB], [http://www.ebi.ac.uk/pdbsum/1jjx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1jjx ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jjx OCA], [https://pdbe.org/1jjx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jjx RCSB], [https://www.ebi.ac.uk/pdbsum/1jjx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jjx ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/FABP7_HUMAN FABP7_HUMAN]] B-FABP could be involved in the transport of a so far unknown hydrophobic ligand with potential morphogenic activity during CNS development. It is required for the establishment of the radial glial fiber system in developing brain, a system that is necessary for the migration of immature neurons to establish cortical layers (By similarity).
+[https://www.uniprot.org/uniprot/FABP7_HUMAN FABP7_HUMAN] B-FABP could be involved in the transport of a so far unknown hydrophobic ligand with potential morphogenic activity during CNS development. It is required for the establishment of the radial glial fiber system in developing brain, a system that is necessary for the migration of immature neurons to establish cortical layers (By similarity).
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 35: / Line 35: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Luecke, C]]
+[[Category: Luecke C]]
-[[Category: Rademacher, M]]
+[[Category: Rademacher M]]
-[[Category: Rueterjans, H]]
+[[Category: Rueterjans H]]
-[[Category: Veerkamp, J H]]
+[[Category: Veerkamp JH]]
-[[Category: Zimmerman, A W]]
+[[Category: Zimmerman AW]]
-[[Category: 15n isotope enrichment]]
-[[Category: Beta barrel]]
-[[Category: Fatty acid carrier]]
-[[Category: Lipid binding protein]]
-[[Category: Nmr spectroscopy]]

1jjx

From Proteopedia

Current revision

Solution Structure of Recombinant Human Brain-type Fatty acid Binding Protein

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox