1jkm

<table><tr><td colspan='2'>[[1jkm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JKM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JKM FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jkm OCA], [http://pdbe.org/1jkm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jkm RCSB], [http://www.ebi.ac.uk/pdbsum/1jkm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1jkm ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Brefeldin A esterase (BFAE), a detoxifying enzyme isolated from Bacillus subtilis, hydrolyzes and inactivates BFA, a potent fungal inhibitor of intracellular vesicle-dependent secretory transport and poliovirus RNA replication. We have solved the crystal structure of BFAE and we discovered that the previously reported amino acid sequence was in serious error due to frame shifts in the cDNA sequence. The correct sequence, inferred from the experimentally phased electron density map, revealed that BFAE is a homolog of the mammalian hormone sensitive lipase (HSL). It is a canonical alpha/beta hydrolase with two insertions forming the substrate binding pocket. The enzyme contains a lipase-like catalytic triad, Ser 202, Asp 308 and His 338, consistent with mutational studies that implicate the homologous Ser 424, Asp 693 and His 723 in the catalytic triad in human HSL.

Crystal structure of brefeldin A esterase, a bacterial homolog of the mammalian hormone-sensitive lipase.,Wei Y, Contreras JA, Sheffield P, Osterlund T, Derewenda U, Kneusel RE, Matern U, Holm C, Derewenda ZS Nat Struct Biol. 1999 Apr;6(4):340-5. PMID:10201402<ref>PMID:10201402</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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== References ==

<references/>

[[Category: Vibrio subtilis ehrenberg 1835]]

[[Category: Contreras, A J]]

[[Category: Derewenda, U]]

[[Category: Derewenda, Z S]]

[[Category: Matern, U O]]

[[Category: Osterlund, T]]

[[Category: Sheffield, P]]

[[Category: Wei, Y]]

[[Category: Serine hydrolase]]