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114d

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[[Image:114d.gif|left|200px]]
 
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==INOSINE-ADENINE BASE PAIRS IN A B-DNA DUPLEX==
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The line below this paragraph, containing "STRUCTURE_114d", creates the "Structure Box" on the page.
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<StructureSection load='114d' size='340' side='right'caption='[[114d]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[114d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=114D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=114D FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=114d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=114d OCA], [https://pdbe.org/114d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=114d RCSB], [https://www.ebi.ac.uk/pdbsum/114d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=114d ProSAT]</span></td></tr>
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{{STRUCTURE_114d| PDB=114d | SCENE= }}
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</table>
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__TOC__
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'''INOSINE-ADENINE BASE PAIRS IN A B-DNA DUPLEX'''
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Brown T]]
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==Overview==
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[[Category: Corfield PWR]]
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The structure of the synthetic deoxydodecamer d(C-G-C-I-A-A-T-T-A-G-C-G) has been determined by single crystal X-ray diffraction techniques at 2.5A resolution. The refinement converged with a crystallographic residual, R = 0.19 and the location of 64 solvent molecules. The sequence crystallises as a B-DNA helix with 10 Watson-Crick base-pairs (4 A.T. and 6 G.C) and 2 inosine.adenine (I.A) pairs. The present work shows that in the purine.purine base-pairs the adenine adopts syn orientation with respect to the furanose moiety while the inosine is in the trans (anti) orientation. Two hydrogen bonds link the I.A. base-pair, one between N-1(I) and N-7(A), the other between O-6(I) and N-6(A). This bulky purine.purine base-pair is incorporated in the double helix at two positions with little distortion of either local or global conformation. The pairing observed in this study is presented as a model for I.A base-pairs in RNA codon-anticodon interactions and may help explain the thermodynamic stability of inosine containing base-pairs. Conformational parameters and base stacking interactions are presented and where appropriate compared with those of the native compound, d(C-G-C-G-A-A-T-T-C-G-C-G) and with other studies of oligonucleotides containing purine.purine base-pairs.
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[[Category: Hunter WN]]
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[[Category: Kennard O]]
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==About this Structure==
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[[Category: Robinson P]]
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=114D OCA].
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==Reference==
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Inosine.adenine base pairs in a B-DNA duplex., Corfield PW, Hunter WN, Brown T, Robinson P, Kennard O, Nucleic Acids Res. 1987 Oct 12;15(19):7935-49. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/3671069 3671069]
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[[Category: Brown, T.]]
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[[Category: Corfield, P W.R.]]
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[[Category: Hunter, W N.]]
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[[Category: Kennard, O.]]
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[[Category: Robinson, P.]]
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[[Category: B-dna]]
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[[Category: Double helix]]
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[[Category: Mismatched]]
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[[Category: Modified]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:26:27 2008''
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INOSINE-ADENINE BASE PAIRS IN A B-DNA DUPLEX

PDB ID 114d

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