131d

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[[Image:131d.jpg|left|200px]]
 
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==THE LOW-TEMPERATURE CRYSTAL STRUCTURE OF THE PURE-SPERMINE FORM OF Z-DNA REVEALS BINDING OF A SPERMINE MOLECULE IN THE MINOR GROOVE==
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The line below this paragraph, containing "STRUCTURE_131d", creates the "Structure Box" on the page.
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<StructureSection load='131d' size='340' side='right'caption='[[131d]], [[Resolution|resolution]] 1.00&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[131d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=131D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=131D FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SPM:SPERMINE'>SPM</scene></td></tr>
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{{STRUCTURE_131d| PDB=131d | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=131d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=131d OCA], [https://pdbe.org/131d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=131d RCSB], [https://www.ebi.ac.uk/pdbsum/131d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=131d ProSAT]</span></td></tr>
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</table>
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'''THE LOW-TEMPERATURE CRYSTAL STRUCTURE OF THE PURE-SPERMINE FORM OF Z-DNA REVEALS BINDING OF A SPERMINE MOLECULE IN THE MINOR GROOVE'''
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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==Overview==
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[[Category: Bancroft D]]
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The X-ray crystal structure of the pure-spermine form of the left-handed Z-DNA duplex [d(CGCGCG)]2 has been determined at a temperature of -110 degrees C. Whereas the previously described room temperature structure of the pure-spermine form showed only the presence of a single "interhelix" spermine molecule, mediating contacts between neighboring duplexes (Egli et al., 1991), a second "intrahelix" spermine molecule as well as two hydrated sodium ions were found in the structure determined at low temperature. This second spermine molecule binds primarily within the minor groove of two hexamer duplexes that are stacked in an end-to-end fashion in the crystal lattice. Thus, the intrahelix spermine molecule interacts with a single infinite helix. The spine of hydration observed in other structures of Z-DNA hexamers is partially replaced and partially displaced by the intrahelix spermine molecule. In Z-DNA, phosphate groups are relatively closely spaced across the minor groove compared to the right-handed double-helical conformation of B-DNA. The intrahelix spermine molecule decreases cross-groove electrostatic repulsion within the Z-DNA helix, thereby increasing its relative stability. This structure may therefore provide an explanation for the role of spermine as a very effective inducer of the conformational B-DNA to Z-DNA transition with alternating dG-dC sequences in solution.
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[[Category: Egli M]]
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[[Category: Rich A]]
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==About this Structure==
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[[Category: Williams LD]]
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=131D OCA].
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==Reference==
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The low-temperature crystal structure of the pure-spermine form of Z-DNA reveals binding of a spermine molecule in the minor groove., Bancroft D, Williams LD, Rich A, Egli M, Biochemistry. 1994 Feb 8;33(5):1073-86. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8110738 8110738]
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[[Category: Bancroft, D.]]
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[[Category: Egli, M.]]
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[[Category: Rich, A.]]
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[[Category: Williams, L D.]]
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[[Category: Double helix]]
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[[Category: Z-dna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:28:46 2008''
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Current revision

THE LOW-TEMPERATURE CRYSTAL STRUCTURE OF THE PURE-SPERMINE FORM OF Z-DNA REVEALS BINDING OF A SPERMINE MOLECULE IN THE MINOR GROOVE

PDB ID 131d

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