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5cv1

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C. elegans PGL-1 Dimerization Domain

Structural highlights

5cv1 is a 1 chain structure with sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.599Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PGL1_CAEEL Essential role in male and female postembryonic germline development; maternally provided protein maintains a population of proliferating germ cells and zygotic expression is required for correct oogenesis. Predicted to bind RNA.^[1]

Publication Abstract from PubMed

Cellular RNA-protein (RNP) granules are ubiquitous and have fundamental roles in biology and RNA metabolism, but the molecular basis of their structure, assembly, and function is poorly understood. Using nematode "P-granules" as a paradigm, we focus on the PGL granule scaffold protein to gain molecular insights into RNP granule structure and assembly. We first identify a PGL dimerization domain (DD) and determine its crystal structure. PGL-1 DD has a novel 13 alpha-helix fold that creates a positively charged channel as a homodimer. We investigate its capacity to bind RNA and discover unexpectedly that PGL-1 DD is a guanosine-specific, single-stranded endonuclease. Discovery of the PGL homodimer, together with previous results, suggests a model in which the PGL DD dimer forms a fundamental building block for P-granule assembly. Discovery of the PGL RNase activity expands the role of RNP granule assembly proteins to include enzymatic activity in addition to their job as structural scaffolds.

PGL germ granule assembly protein is a base-specific, single-stranded RNase.,Aoki ST, Kershner AM, Bingman CA, Wickens M, Kimble J Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201524400. PMID:26787882^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kawasaki I, Shim YH, Kirchner J, Kaminker J, Wood WB, Strome S. PGL-1, a predicted RNA-binding component of germ granules, is essential for fertility in C. elegans. Cell. 1998 Sep 4;94(5):635-45. PMID:9741628
↑ Aoki ST, Kershner AM, Bingman CA, Wickens M, Kimble J. PGL germ granule assembly protein is a base-specific, single-stranded RNase. Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201524400. PMID:26787882 doi:http://dx.doi.org/10.1073/pnas.1524400113

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5cv1"

@@ Line 3: / Line 3: @@
 <StructureSection load='5cv1' size='340' side='right'caption='[[5cv1]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5cv1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CV1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CV1 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5cv1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CV1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CV1 FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5cow|5cow]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.599&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pgl-1, ZK381.4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cv1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cv1 OCA], [https://pdbe.org/5cv1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cv1 RCSB], [https://www.ebi.ac.uk/pdbsum/5cv1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cv1 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cv1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cv1 OCA], [http://pdbe.org/5cv1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cv1 RCSB], [http://www.ebi.ac.uk/pdbsum/5cv1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5cv1 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/PGL1_CAEEL PGL1_CAEEL]] Essential role in male and female postembryonic germline development; maternally provided protein maintains a population of proliferating germ cells and zygotic expression is required for correct oogenesis. Predicted to bind RNA.<ref>PMID:9741628</ref>
+[https://www.uniprot.org/uniprot/PGL1_CAEEL PGL1_CAEEL] Essential role in male and female postembryonic germline development; maternally provided protein maintains a population of proliferating germ cells and zygotic expression is required for correct oogenesis. Predicted to bind RNA.<ref>PMID:9741628</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Caeel]]
+[[Category: Caenorhabditis elegans]]
 [[Category: Large Structures]]
-[[Category: Aoki, S T]]
+[[Category: Aoki ST]]
-[[Category: Bingman, C A]]
+[[Category: Bingman CA]]
-[[Category: Kimble, J E]]
+[[Category: Kimble JE]]
-[[Category: Wickens, M]]
+[[Category: Wickens M]]
-[[Category: Dimer]]
-[[Category: Guanosine endonuclease]]
-[[Category: Hydrolase]]
-[[Category: P-granule]]

5cv1

From Proteopedia

Current revision

C. elegans PGL-1 Dimerization Domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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