5i4a

<table><tr><td colspan='2'>[[5i4a]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Marpk Marpk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I4A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5I4A FirstGlance]. <br>

</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Marpi_0405 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=443254 MARPK])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5i4a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i4a OCA], [http://pdbe.org/5i4a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i4a RCSB], [http://www.ebi.ac.uk/pdbsum/5i4a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5i4a ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Eukaryotic Argonaute proteins induce gene silencing by small RNA-guided recognition and cleavage of mRNA targets. Although structural similarities between human and prokaryotic Argonautes are consistent with shared mechanistic properties, sequence and structure-based alignments suggested that Argonautes encoded within CRISPR-cas[clustered regularly interspaced short palindromic repeats (CRISPR)-associated] bacterial immunity operons have divergent activities. We show here that the CRISPR-associatedMarinitoga piezophilaArgonaute (MpAgo) protein cleaves single-stranded target sequences using 5'-hydroxylated guide RNAs rather than the 5'-phosphorylated guides used by all known Argonautes. The 2.0-A resolution crystal structure of an MpAgo-RNA complex reveals a guide strand binding site comprising residues that block 5' phosphate interactions. Using structure-based sequence alignment, we were able to identify other putative MpAgo-like proteins, all of which are encoded within CRISPR-casloci. Taken together, our data suggest the evolution of an Argonaute subclass with noncanonical specificity for a 5'-hydroxylated guide.

 

A bacterial Argonaute with noncanonical guide RNA specificity.,Kaya E, Doxzen KW, Knoll KR, Wilson RC, Strutt SC, Kranzusch PJ, Doudna JA Proc Natl Acad Sci U S A. 2016 Mar 30. pii: 201524385. PMID:27035975<ref>PMID:27035975</ref>

 

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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== References ==

<references/>

[[Category: Marpk]]

[[Category: Doudna, J A]]

[[Category: Doxzen, K W]]

[[Category: Kaya, E]]

[[Category: Knoll, K R]]

[[Category: Kranzusch, P J]]

[[Category: Strutt, S C]]

[[Category: Wilson, R C]]

[[Category: Rna binding protein-rna complex]]