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| ==Structure of a synthetic beta-carboxysome shell, T=4== | | ==Structure of a synthetic beta-carboxysome shell, T=4== |
- | <StructureSection load='6owg' size='340' side='right'caption='[[6owg]], [[Resolution|resolution]] 2.60Å' scene=''> | + | <SX load='6owg' size='340' side='right' viewer='molstar' caption='[[6owg]], [[Resolution|resolution]] 2.60Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
- | <table><tr><td colspan='2'>[[6owg]] is a 240 chain structure with sequence from [http://en.wikipedia.org/wiki/Aphanothece_halophytica_'solar_lake' Aphanothece halophytica 'solar lake']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OWG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OWG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6owg]] is a 240 chain structure with sequence from [https://en.wikipedia.org/wiki/Halothece_sp._PCC_7418 Halothece sp. PCC 7418]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OWG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OWG FirstGlance]. <br> |
- | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PCC7418_3532 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=65093 Aphanothece halophytica 'Solar Lake']), PCC7418_3533 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=65093 Aphanothece halophytica 'Solar Lake'])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.6Å</td></tr> |
- | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6owg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6owg OCA], [http://pdbe.org/6owg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6owg RCSB], [http://www.ebi.ac.uk/pdbsum/6owg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6owg ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6owg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6owg OCA], [https://pdbe.org/6owg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6owg RCSB], [https://www.ebi.ac.uk/pdbsum/6owg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6owg ProSAT]</span></td></tr> |
| </table> | | </table> |
- | <div style="background-color:#fffaf0;">
| + | == Function == |
- | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/K9YHS7_HALP7 K9YHS7_HALP7] |
- | Carboxysomes are capsid-like, CO2-fixing organelles that are present in all cyanobacteria and some chemoautotrophs and that substantially contribute to global primary production. They are composed of a selectively permeable protein shell that encapsulates Rubisco, the principal CO2-fixing enzyme, and carbonic anhydrase. As the centerpiece of the carbon-concentrating mechanism, by packaging enzymes that collectively enhance catalysis, the carboxysome shell enables the generation of a locally elevated concentration of substrate CO2 and the prevention of CO2 escape. A functional carboxysome consisting of an intact shell and cargo is essential for cyanobacterial growth under ambient CO2 concentrations. Using cryo-electron microscopy, we have determined the structure of a recombinantly produced simplified beta-carboxysome shell. The structure reveals the sidedness and the specific interactions between the carboxysome shell proteins. The model provides insight into the structural basis of selective permeability of the carboxysome shell and can be used to design modifications to investigate the mechanisms of cargo encapsulation and other physiochemical properties such as permeability. Notably, the permeability properties are of great interest for modeling and evaluating this carbon-concentrating mechanism in metabolic engineering. Moreover, we find striking similarity between the carboxysome shell and the structurally characterized, evolutionarily distant metabolosome shell, implying universal architectural principles for bacterial microcompartment shells.
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- | Structure of a Synthetic beta-Carboxysome Shell.,Sutter M, Laughlin TG, Sloan NB, Serwas D, Davies KM, Kerfeld CA Plant Physiol. 2019 Nov;181(3):1050-1058. doi: 10.1104/pp.19.00885. Epub 2019 Sep, 9. PMID:31501298<ref>PMID:31501298</ref>
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- | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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- | </div>
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- | <div class="pdbe-citations 6owg" style="background-color:#fffaf0;"></div>
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- | == References ==
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- | <references/>
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| __TOC__ | | __TOC__ |
- | </StructureSection> | + | </SX> |
- | [[Category: Aphanothece halophytica 'solar lake']] | + | [[Category: Halothece sp. PCC 7418]] |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
- | [[Category: Davies, K M]] | + | [[Category: Davies KM]] |
- | [[Category: Kerfeld, C A]] | + | [[Category: Kerfeld CA]] |
- | [[Category: Laughlin, T G]] | + | [[Category: Laughlin TG]] |
- | [[Category: Sutter, M]] | + | [[Category: Sutter M]] |
- | [[Category: Bacterial microcompartment]]
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- | [[Category: Carboxysome]]
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- | [[Category: Structural protein]]
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