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| | ==1H NMR STUDY OF THE SOLUTION STRUCTURE OF AC-AMP2== | | ==1H NMR STUDY OF THE SOLUTION STRUCTURE OF AC-AMP2== |
| - | <StructureSection load='1mmc' size='340' side='right'caption='[[1mmc]], [[NMR_Ensembles_of_Models | 26 NMR models]]' scene=''> | + | <StructureSection load='1mmc' size='340' side='right'caption='[[1mmc]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1mmc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Amaranthus_caudatus Amaranthus caudatus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MMC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MMC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1mmc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Amaranthus_caudatus Amaranthus caudatus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MMC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MMC FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mmc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mmc OCA], [http://pdbe.org/1mmc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mmc RCSB], [http://www.ebi.ac.uk/pdbsum/1mmc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mmc ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 26 models</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mmc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mmc OCA], [https://pdbe.org/1mmc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mmc RCSB], [https://www.ebi.ac.uk/pdbsum/1mmc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mmc ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/AMP_AMACA AMP_AMACA]] Chitin-binding protein with a defensive function against numerous chitin containing fungal pathogens. It is also a potent inhibitor of Gram-positive bacteria. | + | [https://www.uniprot.org/uniprot/AMP_AMACA AMP_AMACA] Chitin-binding protein with a defensive function against numerous chitin containing fungal pathogens. It is also a potent inhibitor of Gram-positive bacteria. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | [[Category: Amaranthus caudatus]] | | [[Category: Amaranthus caudatus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Loris, R]] | + | [[Category: Loris R]] |
| - | [[Category: Maes, D]] | + | [[Category: Maes D]] |
| - | [[Category: Martins, J C]] | + | [[Category: Martins JC]] |
| - | [[Category: Pepermans, H A.M]] | + | [[Category: Pepermans HAM]] |
| - | [[Category: Verheyden, P]] | + | [[Category: Verheyden P]] |
| - | [[Category: Willem, R]] | + | [[Category: Willem R]] |
| - | [[Category: Wyns, L]] | + | [[Category: Wyns L]] |
| - | [[Category: Antifungal antimicrobial]]
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| - | [[Category: Chitin-binding]]
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| Structural highlights
Function
AMP_AMACA Chitin-binding protein with a defensive function against numerous chitin containing fungal pathogens. It is also a potent inhibitor of Gram-positive bacteria.
Publication Abstract from PubMed
The conformation in water of antimicrobial protein 2 from Amaranthus caudatus (Ac-AMP2) was determined using 1H NMR, DIANA and restrained molecular modeling. Ac-AMP2 is a 30 amino acid residue, lectin-like protein that specifically binds to chitin, a polymer of beta-1,4-N-acetyl-D-glucosamine. After sequence specific resonance assignments, a total of 198 distance restraints were collected from 2D NOESY buildup spectra at 500 MHz at pH 2, supplemented by a 2D NOESY spectrum at 600 MHz. The location of the three previously unassigned disulfide bridges was determined from preliminary DIANA structures, using a statistical analysis of intercystinyl distances. The solution structure of Ac-AMP2 is presented as a set of 26 DIANA structures, further refined by restrained molecular dynamics using a simulated annealing protocol in the AMBER force field, with a backbone r.m.s.d. for the well defined Glu3-Cys28 segment of 0.69(+/-0.12) angstroms. The main structural element is an antiparallel beta-sheet from Met13 to Lys23 including a betaI-turn over Gln17-Phel8 with a beta bulge at Gly19. In addition, a beta'I turn over Arg6-Gly7, a beta'III turn over Ser11-Gly12 and a helical turn from Gly24 to Cys28 are identified. This structure is very similar to the equivalent regions of the X-ray structure of wheat germ agglutinin and the NMR structure of hevein.
H NMR study of the solution structure of Ac-AMP2, a sugar binding antimicrobial protein isolated from Amaranthus caudatus.,Martins JC, Maes D, Loris R, Pepermans HA, Wyns L, Willem R, Verheyden P J Mol Biol. 1996 May 3;258(2):322-33. PMID:8627629[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Martins JC, Maes D, Loris R, Pepermans HA, Wyns L, Willem R, Verheyden P. H NMR study of the solution structure of Ac-AMP2, a sugar binding antimicrobial protein isolated from Amaranthus caudatus. J Mol Biol. 1996 May 3;258(2):322-33. PMID:8627629
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