1n4n

From Proteopedia

(Difference between revisions)

Current revision

Structure of the Plant Defensin PhD1 from Petunia Hybrida

Structural highlights

1n4n is a 1 chain structure with sequence from Petunia x hybrida. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR, 20 models
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DEF1_PETHY Plant defense peptide with antifungal activity against F.oxysporum and B.cinerea.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The structure of a novel plant defensin isolated from the flowers of Petunia hybrida has been determined by (1)H NMR spectroscopy. P. hybrida defensin 1 (PhD1) is a basic, cysteine-rich, antifungal protein of 47 residues and is the first example of a new subclass of plant defensins with five disulfide bonds whose structure has been determined. PhD1 has the fold of the cysteine-stabilized alphabeta motif, consisting of an alpha-helix and a triple-stranded antiparallel beta-sheet, except that it contains a fifth disulfide bond from the first loop to the alpha-helix. The additional disulfide bond is accommodated in PhD1 without any alteration of its tertiary structure with respect to other plant defensins. Comparison of its structure with those of classic, four-disulfide defensins has allowed us to identify a previously unrecognized hydrogen bond network that is integral to structure stabilization in the family.

Structure of Petunia hybrida defensin 1, a novel plant defensin with five disulfide bonds.,Janssen BJ, Schirra HJ, Lay FT, Anderson MA, Craik DJ Biochemistry. 2003 Jul 15;42(27):8214-22. PMID:12846570^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Janssen BJ, Schirra HJ, Lay FT, Anderson MA, Craik DJ. Structure of Petunia hybrida defensin 1, a novel plant defensin with five disulfide bonds. Biochemistry. 2003 Jul 15;42(27):8214-22. PMID:12846570 doi:10.1021/bi034379o

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1n4n"

@@ Line 1: / Line 1: @@
 ==Structure of the Plant Defensin PhD1 from Petunia Hybrida==
-<StructureSection load='1n4n' size='340' side='right'caption='[[1n4n]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='1n4n' size='340' side='right'caption='[[1n4n]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1n4n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Petunia_x_hybrida Petunia x hybrida]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N4N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N4N FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1n4n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Petunia_x_hybrida Petunia x hybrida]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N4N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N4N FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n4n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n4n OCA], [http://pdbe.org/1n4n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1n4n RCSB], [http://www.ebi.ac.uk/pdbsum/1n4n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1n4n ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n4n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n4n OCA], [https://pdbe.org/1n4n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n4n RCSB], [https://www.ebi.ac.uk/pdbsum/1n4n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n4n ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/DEF1_PETHY DEF1_PETHY]] Plant defense peptide with antifungal activity against F.oxysporum and B.cinerea.
+[https://www.uniprot.org/uniprot/DEF1_PETHY DEF1_PETHY] Plant defense peptide with antifungal activity against F.oxysporum and B.cinerea.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 13: / Line 14: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n4/1n4n_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 36: / Line 37: @@
 [[Category: Large Structures]]
 [[Category: Petunia x hybrida]]
-[[Category: Anderson, M A]]
+[[Category: Anderson MA]]
-[[Category: Craik, D J]]
+[[Category: Craik DJ]]
-[[Category: Janssen, B J.C]]
+[[Category: Janssen BJC]]
-[[Category: Lay, F T]]
+[[Category: Lay FT]]
-[[Category: Schirra, H J]]
+[[Category: Schirra HJ]]
-[[Category: Cysteine-stabilised alpha-beta motif]]
-[[Category: Fifth disulfide bond]]
-[[Category: Plant protein]]

1n4n

From Proteopedia

Current revision

Structure of the Plant Defensin PhD1 from Petunia Hybrida

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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