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6ozx

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Current revision (14:53, 13 March 2024) (edit) (undo)
 
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<StructureSection load='6ozx' size='340' side='right'caption='[[6ozx]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
<StructureSection load='6ozx' size='340' side='right'caption='[[6ozx]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6ozx]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OZX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OZX FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6ozx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caulobacter_vibrioides_CB15 Caulobacter vibrioides CB15]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OZX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OZX FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ozx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ozx OCA], [http://pdbe.org/6ozx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ozx RCSB], [http://www.ebi.ac.uk/pdbsum/6ozx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ozx ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.851&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ozx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ozx OCA], [https://pdbe.org/6ozx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ozx RCSB], [https://www.ebi.ac.uk/pdbsum/6ozx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ozx ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Y3319_CAUVC Y3319_CAUVC]
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GapR is a nucleoid-associated protein required for the cell cycle of Caulobacter cresentus. We have determined new crystal structures of GapR to high resolution. As in a recently published structure, a GapR monomer folds into one long N-terminal alpha helix and two shorter alpha helices, and assembles into a tetrameric ring with a closed, positively charged, central channel. In contrast to the conclusions drawn from the published structures, we observe that the central channel of the tetramer presented here could freely accommodate B-DNA. Mutation of six conserved lysine residues lining the cavity and electrophoretic mobility gel shift experiments confirmed their role in DNA binding and the channel as the site of DNA binding. Although present in our crystals, DNA could not be observed in the electron density maps, suggesting that DNA binding is non-specific, which could be important for tetramer-ring translocation along the chromosome. In conjunction with previous GapR structures we propose a model for DNA binding and translocation that explains key published observations on GapR and its biological functions.
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Structures of GapR reveal a central channel which could accommodate B-DNA.,Tarry MJ, Harmel C, Taylor JA, Marczynski GT, Schmeing TM Sci Rep. 2019 Nov 13;9(1):16679. doi: 10.1038/s41598-019-52964-2. PMID:31723182<ref>PMID:31723182</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6ozx" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Caulobacter vibrioides CB15]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Harmel, C]]
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[[Category: Harmel C]]
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[[Category: Marczynski, G T]]
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[[Category: Marczynski GT]]
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[[Category: Schmeing, T M]]
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[[Category: Schmeing TM]]
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[[Category: Tarry, M]]
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[[Category: Tarry M]]
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[[Category: Taylor, J A]]
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[[Category: Taylor JA]]
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[[Category: Cell-division]]
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[[Category: Dna binding protein]]
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[[Category: Dna-binding]]
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Current revision

Wild type GapR crystal structure 1 from C. crescentus

PDB ID 6ozx

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