6uro
From Proteopedia
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==Cryo-EM structure of human CPSF160-WDR33-CPSF30-PAS RNA-CstF77 complex== | ==Cryo-EM structure of human CPSF160-WDR33-CPSF30-PAS RNA-CstF77 complex== | ||
| - | < | + | <SX load='6uro' size='340' side='right' viewer='molstar' caption='[[6uro]], [[Resolution|resolution]] 3.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6uro]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6URO OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[6uro]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Macaca_mulatta_polyomavirus_1 Macaca mulatta polyomavirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6URO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6URO FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6uro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uro OCA], [https://pdbe.org/6uro PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6uro RCSB], [https://www.ebi.ac.uk/pdbsum/6uro PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6uro ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/CPSF1_HUMAN CPSF1_HUMAN] Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.<ref>PMID:14749727</ref> |
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The mammalian pre-mRNA 3'-end-processing machinery consists of cleavage and polyadenylation specificity factor (CPSF), cleavage stimulation factor (CstF), and other proteins, but the overall architecture of this machinery remains unclear. CPSF contains two functionally distinct modules: a cleavage factor (mCF) and a polyadenylation specificity factor (mPSF). Here, we have produced recombinant human CPSF and CstF and examined these factors by electron microscopy (EM). We find that mPSF is the organizational core of the machinery, while the conformations of mCF and CstF and the position of mCF relative to mPSF are highly variable. We have identified by cryo-EM a segment in CPSF100 that tethers mCF to mPSF, and we have named it the PSF interaction motif (PIM). Mutations in the PIM can abolish CPSF formation, indicating that it is a crucial contact in CPSF. We have also obtained reconstructions of mCF and CstF77 by cryo-EM, assembled around the mPSF core. | ||
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| + | Structural Insights into the Human Pre-mRNA 3'-End Processing Machinery.,Zhang Y, Sun Y, Shi Y, Walz T, Tong L Mol Cell. 2019 Nov 25. pii: S1097-2765(19)30834-2. doi:, 10.1016/j.molcel.2019.11.005. PMID:31810758<ref>PMID:31810758</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6uro" style="background-color:#fffaf0;"></div> | ||
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| + | ==See Also== | ||
| + | *[[Poly(ADP-ribose) polymerase 3D structures|Poly(ADP-ribose) polymerase 3D structures]] | ||
| + | *[[WD-repeat protein 3D structures|WD-repeat protein 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
| - | </ | + | </SX> |
| + | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Sun | + | [[Category: Macaca mulatta polyomavirus 1]] |
| - | [[Category: Tong | + | [[Category: Sun Y]] |
| - | [[Category: Walz | + | [[Category: Tong L]] |
| - | [[Category: Zhang | + | [[Category: Walz T]] |
| - | + | [[Category: Zhang Y]] | |
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Current revision
Cryo-EM structure of human CPSF160-WDR33-CPSF30-PAS RNA-CstF77 complex
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