1qgv

From Proteopedia

(Difference between revisions)

Current revision

HUMAN SPLICEOSOMAL PROTEIN U5-15KD

Structural highlights

1qgv is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.4Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TXN4A_HUMAN Essential role in pre-mRNA splicing.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The U5 small ribonucleoprotein particle (snRNP) contains various proteins involved in catalytic activities mediating conformational rearrangements of the spliceosome. We have isolated and characterized the evolutionarily highly conserved human U5 snRNP-specific protein U5-15kD. The crystal structure of U5-15kD determined at 1.4 A resolution revealed a thioredoxin-like fold and represents the first structure of a U5 snRNP-specific protein known so far. With respect to human thioredoxin the U5-15kD protein contains 37 additional residues causing structural changes which most likely form putative binding sites for other spliceosomal proteins or RNA. Moreover, a novel intramolecular disulfide bond replaces the canonical one found in the thioredoxin family. Even though U5-15kD appears to lack protein disulfide isomerase activity, it is strictly required for pre-mRNA splicing in vivo as we demonstrate by genetic depletion of its ortholog in Saccharomyces cerevisiae. Our data suggest that the previously reported involvement of its Schizosaccharomyces pombe ortholog Dim1p in cell cycle regulation is a consequence of its essential role in pre-mRNA splicing.

Identification, characterization and crystal structure analysis of the human spliceosomal U5 snRNP-specific 15 kD protein.,Reuter K, Nottrott S, Fabrizio P, Luhrmann R, Ficner R J Mol Biol. 1999 Nov 26;294(2):515-25. PMID:10610776^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Reuter K, Nottrott S, Fabrizio P, Luhrmann R, Ficner R. Identification, characterization and crystal structure analysis of the human spliceosomal U5 snRNP-specific 15 kD protein. J Mol Biol. 1999 Nov 26;294(2):515-25. PMID:10610776 doi:10.1006/jmbi.1999.3258

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1qgv"

@@ Line 3: / Line 3: @@
 <StructureSection load='1qgv' size='340' side='right'caption='[[1qgv]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1qgv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QGV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QGV FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1qgv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QGV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QGV FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">U5-15KD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qgv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qgv OCA], [http://pdbe.org/1qgv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qgv RCSB], [http://www.ebi.ac.uk/pdbsum/1qgv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1qgv ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qgv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qgv OCA], [https://pdbe.org/1qgv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qgv RCSB], [https://www.ebi.ac.uk/pdbsum/1qgv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qgv ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/TXN4A_HUMAN TXN4A_HUMAN]] Essential role in pre-mRNA splicing.
+[https://www.uniprot.org/uniprot/TXN4A_HUMAN TXN4A_HUMAN] Essential role in pre-mRNA splicing.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 14: / Line 14: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qg/1qgv_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 30: / Line 30: @@
 ==See Also==
-*[[HDim1/U5-15kD|HDim1/U5-15kD]]
 *[[U5-15kD|U5-15kD]]
-*[[User:John S. de Banzie/Globular Protein|User:John S. de Banzie/Globular Protein]]
-*[[User:John S. de Banzie/GlobularProtein|User:John S. de Banzie/GlobularProtein]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Fabrizio, P]]
+[[Category: Fabrizio P]]
-[[Category: Ficner, R]]
+[[Category: Ficner R]]
-[[Category: Luehrmann, R]]
+[[Category: Luehrmann R]]
-[[Category: Nottrott, S]]
+[[Category: Nottrott S]]
-[[Category: Reuter, K]]
+[[Category: Reuter K]]
-[[Category: Snrnp]]
-[[Category: Spliceosomal protein]]
-[[Category: Thioredoxin]]
-[[Category: Transcription]]

1qgv

From Proteopedia

Current revision

HUMAN SPLICEOSOMAL PROTEIN U5-15KD

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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