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| | ==CryoEM Structure of a Prokaryotic Cyclic Nucleotide-Gated Ion Channel== | | ==CryoEM Structure of a Prokaryotic Cyclic Nucleotide-Gated Ion Channel== |
| - | <StructureSection load='5v4s' size='340' side='right'caption='[[5v4s]], [[Resolution|resolution]] 4.20Å' scene=''> | + | <SX load='5v4s' size='340' side='right' viewer='molstar' caption='[[5v4s]], [[Resolution|resolution]] 4.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5v4s]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Leptospira_licerasiae_serovar_varillal_str._var_010 Leptospira licerasiae serovar varillal str. var 010]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V4S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5V4S FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5v4s]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Leptospira_licerasiae_serovar_Varillal_str._VAR_010 Leptospira licerasiae serovar Varillal str. VAR 010]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V4S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5V4S FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LEP1GSC185_1946 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1049972 Leptospira licerasiae serovar Varillal str. VAR 010])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.2Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5v4s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5v4s OCA], [http://pdbe.org/5v4s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5v4s RCSB], [http://www.ebi.ac.uk/pdbsum/5v4s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5v4s ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5v4s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5v4s OCA], [https://pdbe.org/5v4s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5v4s RCSB], [https://www.ebi.ac.uk/pdbsum/5v4s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5v4s ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/I0XVQ9_9LEPT I0XVQ9_9LEPT] |
| - | Cyclic nucleotide-gated (CNG) and hyperpolarization-activated cyclic nucleotide-regulated (HCN) ion channels play crucial physiological roles in phototransduction, olfaction, and cardiac pace making. These channels are characterized by the presence of a carboxyl-terminal cyclic nucleotide-binding domain (CNBD) that connects to the channel pore via a C-linker domain. Although cyclic nucleotide binding has been shown to promote CNG and HCN channel opening, the precise mechanism underlying gating remains poorly understood. Here we used cryoEM to determine the structure of the intact LliK CNG channel isolated from Leptospira licerasiae-which shares sequence similarity to eukaryotic CNG and HCN channels-in the presence of a saturating concentration of cAMP. A short S4-S5 linker connects nearby voltage-sensing and pore domains to produce a non-domain-swapped transmembrane architecture, which appears to be a hallmark of this channel family. We also observe major conformational changes of the LliK C-linkers and CNBDs relative to the crystal structures of isolated C-linker/CNBD fragments and the cryoEM structures of related CNG, HCN, and KCNH channels. The conformation of our LliK structure may represent a functional state of this channel family not captured in previous studies.
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| - | CryoEM structure of a prokaryotic cyclic nucleotide-gated ion channel.,James ZM, Borst AJ, Haitin Y, Frenz B, DiMaio F, Zagotta WN, Veesler D Proc Natl Acad Sci U S A. 2017 Apr 25;114(17):4430-4435. doi:, 10.1073/pnas.1700248114. Epub 2017 Apr 10. PMID:28396445<ref>PMID:28396445</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 5v4s" style="background-color:#fffaf0;"></div>
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| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| - | </StructureSection> | + | </SX> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Leptospira licerasiae serovar varillal str. var 010]] | + | [[Category: Leptospira licerasiae serovar Varillal str. VAR 010]] |
| - | [[Category: Borst, A J]] | + | [[Category: Borst AJ]] |
| - | [[Category: DiMaio, F]] | + | [[Category: DiMaio F]] |
| - | [[Category: Frenz, B]] | + | [[Category: Frenz B]] |
| - | [[Category: Haitin, Y]] | + | [[Category: Haitin Y]] |
| - | [[Category: James, Z M]] | + | [[Category: James ZM]] |
| - | [[Category: Veesler, D]] | + | [[Category: Veesler D]] |
| - | [[Category: Zagotta, W N]] | + | [[Category: Zagotta WN]] |
| - | [[Category: Allostery]]
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| - | [[Category: Cyclic nucleotide]]
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| - | [[Category: Ion channel]]
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| - | [[Category: Olfaction]]
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| - | [[Category: Transport protein]]
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| - | [[Category: Vision]]
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