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| | <StructureSection load='6dql' size='340' side='right'caption='[[6dql]], [[Resolution|resolution]] 3.30Å' scene=''> | | <StructureSection load='6dql' size='340' side='right'caption='[[6dql]], [[Resolution|resolution]] 3.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6dql]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_scarlatinae"_klein_1884 "micrococcus scarlatinae" klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DQL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DQL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6dql]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DQL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DQL FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rgg ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1314 "Micrococcus scarlatinae" Klein 1884])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dql FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dql OCA], [http://pdbe.org/6dql PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dql RCSB], [http://www.ebi.ac.uk/pdbsum/6dql PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dql ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dql FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dql OCA], [https://pdbe.org/6dql PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dql RCSB], [https://www.ebi.ac.uk/pdbsum/6dql PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dql ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/D3KVD8_STRPY D3KVD8_STRPY] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Micrococcus scarlatinae klein 1884]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Do, H]] | + | [[Category: Streptococcus pyogenes]] |
| - | [[Category: Kumaraswami, M]] | + | [[Category: Do H]] |
| - | [[Category: Makthal, N]] | + | [[Category: Kumaraswami M]] |
| - | [[Category: Musser, J M]] | + | [[Category: Makthal N]] |
| - | [[Category: Olsen, R J]] | + | [[Category: Musser JM]] |
| - | [[Category: VanderWal, A R]] | + | [[Category: Olsen RJ]] |
| - | [[Category: Dna binding protein]]
| + | [[Category: VanderWal AR]] |
| - | [[Category: Quorum sensing]]
| + | |
| - | [[Category: Regulator]]
| + | |
| - | [[Category: Streptococcus pyogene]]
| + | |
| - | [[Category: Virulence regulation]]
| + | |
| Structural highlights
Function
D3KVD8_STRPY
Publication Abstract from PubMed
Bacteria control gene expression in concert with their population density by a process called quorum sensing, which is modulated by bacterial chemical signals and environmental factors. In the human pathogen Streptococcus pyogenes, production of secreted virulence factor SpeB is controlled by a quorum-sensing pathway and environmental pH. The quorum-sensing pathway consists of a secreted leaderless peptide signal (SIP), and its cognate receptor RopB. Here, we report that the SIP quorum-sensing pathway has a pH-sensing mechanism operative through a pH-sensitive histidine switch located at the base of the SIP-binding pocket of RopB. Environmental acidification induces protonation of His144 and reorganization of hydrogen bonding networks in RopB, which facilitates SIP recognition. The convergence of two disparate signals in the SIP signaling pathway results in induction of SpeB production and increased bacterial virulence. Our findings provide a model for investigating analogous crosstalk in other microorganisms.
Environmental pH and peptide signaling control virulence of Streptococcus pyogenes via a quorum-sensing pathway.,Do H, Makthal N, VanderWal AR, Saavedra MO, Olsen RJ, Musser JM, Kumaraswami M Nat Commun. 2019 Jun 13;10(1):2586. doi: 10.1038/s41467-019-10556-8. PMID:31197146[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Do H, Makthal N, VanderWal AR, Saavedra MO, Olsen RJ, Musser JM, Kumaraswami M. Environmental pH and peptide signaling control virulence of Streptococcus pyogenes via a quorum-sensing pathway. Nat Commun. 2019 Jun 13;10(1):2586. doi: 10.1038/s41467-019-10556-8. PMID:31197146 doi:http://dx.doi.org/10.1038/s41467-019-10556-8
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