1pvi

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<StructureSection load='1pvi' size='340' side='right'caption='[[1pvi]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
<StructureSection load='1pvi' size='340' side='right'caption='[[1pvi]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[1pvi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Proteus_vulgaris Proteus vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PVI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PVI FirstGlance]. <br>
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<table><tr><td colspan='2'>[[1pvi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Proteus_vulgaris Proteus vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PVI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PVI FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pvi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pvi OCA], [http://pdbe.org/1pvi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1pvi RCSB], [http://www.ebi.ac.uk/pdbsum/1pvi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1pvi ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pvi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pvi OCA], [https://pdbe.org/1pvi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pvi RCSB], [https://www.ebi.ac.uk/pdbsum/1pvi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pvi ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/T2P2_PROHU T2P2_PROHU]] Recognizes the double-stranded sequence CAGCTG and cleaves after G-3.
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[https://www.uniprot.org/uniprot/T2P2_PROHU T2P2_PROHU] Recognizes the double-stranded sequence CAGCTG and cleaves after G-3.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We have determined the structure of PvuII endonuclease complexed with cognate DNA by X-ray crystallography. The DNA substrate is bound with a single homodimeric protein, each subunit of which reveals three structural regions. The catalytic region strongly resembles structures of other restriction endonucleases, even though these regions have dissimilar primary sequences. Comparison of the active site with those of EcoRV and EcoRI endonucleases reveals a conserved triplet sequence close to the reactive phosphodiester group and a conserved acidic pair that may represent the ligands for the catalytic cofactor Mg2+. The DNA duplex is not significantly bent and maintains a B-DNA-like conformation. The subunit interface region of the homodimeric protein consists of a pseudo-three-helix bundle. Direct contacts between the protein and the base pairs of the PvuII recognition site occur exclusively in the major groove through two antiparallel beta strands from the sequence recognition region of the protein. Water-mediated contacts are made in the minor grooves to central bases of the site. If restriction enzymes do share a common ancestor, as has been proposed, their catalytic regions have been very strongly conserved, while their subunit interfaces and DNA sequence recognition regions have undergone remarkable structural variation.
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Structure of PvuII endonuclease with cognate DNA.,Cheng X, Balendiran K, Schildkraut I, Anderson JE EMBO J. 1994 Sep 1;13(17):3927-35. PMID:8076590<ref>PMID:8076590</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1pvi" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Proteus vulgaris]]
[[Category: Proteus vulgaris]]
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[[Category: Anderson, J E]]
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[[Category: Anderson JE]]
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[[Category: Balendiran, K]]
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[[Category: Balendiran K]]
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[[Category: Cheng, X]]
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[[Category: Cheng X]]
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[[Category: Schildkraut, I]]
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[[Category: Schildkraut I]]
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[[Category: Hydrolase-dna complex]]
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[[Category: Protein-dna complex]]
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Current revision

STRUCTURE OF PVUII ENDONUCLEASE WITH COGNATE DNA

PDB ID 1pvi

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