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| | <StructureSection load='1osy' size='340' side='right'caption='[[1osy]], [[Resolution|resolution]] 1.70Å' scene=''> | | <StructureSection load='1osy' size='340' side='right'caption='[[1osy]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1osy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Flammulina_velutipes Flammulina velutipes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OSY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OSY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1osy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Flammulina_velutipes Flammulina velutipes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OSY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OSY FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1osy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1osy OCA], [http://pdbe.org/1osy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1osy RCSB], [http://www.ebi.ac.uk/pdbsum/1osy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1osy ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1osy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1osy OCA], [https://pdbe.org/1osy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1osy RCSB], [https://www.ebi.ac.uk/pdbsum/1osy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1osy ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/FVE_FLAVE FVE_FLAVE]] Lectin with specificity for complex cell-surface carbohydrates. Possesses immunomodulatory activity, stimulates lymphocyte mitogenesis, suppresses systemic anaphylaxis reactions and edema, enhances transcription of IL-2, IFN-gamma and TNF-alpha and hemagglutinates red blood cells. | + | [https://www.uniprot.org/uniprot/FVE_FLAVE FVE_FLAVE] Lectin with specificity for complex cell-surface carbohydrates. Possesses immunomodulatory activity, stimulates lymphocyte mitogenesis, suppresses systemic anaphylaxis reactions and edema, enhances transcription of IL-2, IFN-gamma and TNF-alpha and hemagglutinates red blood cells. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | [[Category: Flammulina velutipes]] | | [[Category: Flammulina velutipes]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Chua, K Y]] | + | [[Category: Chua KY]] |
| - | [[Category: Joseph, J S]] | + | [[Category: Joseph JS]] |
| - | [[Category: Kolatkar, P R]] | + | [[Category: Kolatkar PR]] |
| - | [[Category: Palasingam, P]] | + | [[Category: Palasingam P]] |
| - | [[Category: Robinson, H]] | + | [[Category: Robinson H]] |
| - | [[Category: Seow, S V]] | + | [[Category: Seow SV]] |
| - | [[Category: Shai, V]] | + | [[Category: Shai V]] |
| - | [[Category: Fibronectin fold]]
| + | |
| - | [[Category: Fungal protein]]
| + | |
| - | [[Category: Hemagglutination]]
| + | |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Immunomodulatory]]
| + | |
| - | [[Category: Lectin]]
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| - | [[Category: Sugar binding protein]]
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| Structural highlights
Function
FVE_FLAVE Lectin with specificity for complex cell-surface carbohydrates. Possesses immunomodulatory activity, stimulates lymphocyte mitogenesis, suppresses systemic anaphylaxis reactions and edema, enhances transcription of IL-2, IFN-gamma and TNF-alpha and hemagglutinates red blood cells.
Publication Abstract from PubMed
Fve, a major fruiting body protein from Flammulina velutipes, a mushroom possessing immunomodulatory activity, stimulates lymphocyte mitogenesis, suppresses systemic anaphylaxis reactions and edema, enhances transcription of IL-2, IFN-gamma and TNF-alpha, and hemagglutinates red blood cells. It appears to be a lectin with specificity for complex cell-surface carbohydrates. Fve is a non-covalently linked homodimer containing no Cys, His or Met residues. It shares sequence similarity only to the other fungal immunomodulatory proteins (FIPs) LZ-8, Gts, Vvo and Vvl, all of unknown structure. The 1.7A structure of Fve solved by single anomalous diffraction of NaBr-soaked crystals is novel: each monomer consists of an N-terminal alpha-helix followed by a fibronectin III (FNIII) fold. The FNIII fold is the first instance of "pseudo-h-type" topology, a transition between the seven beta-stranded s-type and the eight beta-stranded h-type topologies. The structure suggests that dimerization, critical for the activity of FIPs, occurs by 3-D domain swapping of the N-terminal helices and is stabilized predominantly by hydrophobic interactions. The structure of Fve is the first in this lectin family to be reported, and the first of an FNIII domain-containing protein of fungal origin.
A 1.7A structure of Fve, a member of the new fungal immunomodulatory protein family.,Paaventhan P, Joseph JS, Seow SV, Vaday S, Robinson H, Chua KY, Kolatkar PR J Mol Biol. 2003 Sep 12;332(2):461-70. PMID:12948495[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Paaventhan P, Joseph JS, Seow SV, Vaday S, Robinson H, Chua KY, Kolatkar PR. A 1.7A structure of Fve, a member of the new fungal immunomodulatory protein family. J Mol Biol. 2003 Sep 12;332(2):461-70. PMID:12948495
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