1a0a

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[[Image:1a0a.gif|left|200px]]
 
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==PHOSPHATE SYSTEM POSITIVE REGULATORY PROTEIN PHO4/DNA COMPLEX==
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The line below this paragraph, containing "STRUCTURE_1a0a", creates the "Structure Box" on the page.
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<StructureSection load='1a0a' size='340' side='right'caption='[[1a0a]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1a0a]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A0A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A0A FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a0a OCA], [https://pdbe.org/1a0a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a0a RCSB], [https://www.ebi.ac.uk/pdbsum/1a0a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a0a ProSAT]</span></td></tr>
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{{STRUCTURE_1a0a| PDB=1a0a | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PHO4_YEAST PHO4_YEAST] Transcriptional activator that regulates the expression of repressible phosphatase under phosphate starvation conditions. Binds to the upstream activating sequence (UAS) of several phosphatase encoding PHO genes. Inhibited by the cyclin-CDK PHO80-PHO85 under high-phosphate conditions.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a0/1a0a_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a0a ConSurf].
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<div style="clear:both"></div>
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'''PHOSPHATE SYSTEM POSITIVE REGULATORY PROTEIN PHO4/DNA COMPLEX'''
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==See Also==
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*[[Pho4|Pho4]]
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__TOC__
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==Overview==
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</StructureSection>
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The crystal structure of a DNA-binding domain of PHO4 complexed with DNA at 2.8 A resolution revealed that the domain folds into a basic-helix-loop-helix (bHLH) motif with a long but compact loop that contains a short alpha-helical segment. This helical structure positions a tryptophan residue into an aromatic cluster so as to make the loop compact. PHO4 binds to DNA as a homodimer with direct reading of both the core E-box sequence CACGTG and its 3'-flanking bases. The 3'-flanking bases GG are recognized by Arg2 and His5. The residues involved in the E-box recognition are His5, Glu9 and Arg13, as already reported for bHLH/Zip proteins MAX and USF, and are different from those recognized by bHLH proteins MyoD and E47, although PHO4 is a bHLH protein.
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[[Category: Large Structures]]
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==About this Structure==
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1A0A is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A0A OCA].
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==Reference==
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Crystal structure of PHO4 bHLH domain-DNA complex: flanking base recognition., Shimizu T, Toumoto A, Ihara K, Shimizu M, Kyogoku Y, Ogawa N, Oshima Y, Hakoshima T, EMBO J. 1997 Aug 1;16(15):4689-97. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9303313 9303313]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Hakoshima T]]
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[[Category: Hakoshima, T.]]
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[[Category: Ihara K]]
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[[Category: Ihara, K.]]
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[[Category: Kyogoku Y]]
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[[Category: Kyogoku, Y.]]
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[[Category: Ogawa N]]
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[[Category: Ogawa, N.]]
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[[Category: Oshima Y]]
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[[Category: Oshima, Y.]]
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[[Category: Shimizu M]]
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[[Category: Shimizu, M.]]
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[[Category: Shimizu T]]
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[[Category: Shimizu, T.]]
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[[Category: Toumoto A]]
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[[Category: Toumoto, A.]]
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[[Category: Basic helix loop helix]]
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[[Category: Transcription factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:36:57 2008''
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Current revision

PHOSPHATE SYSTEM POSITIVE REGULATORY PROTEIN PHO4/DNA COMPLEX

PDB ID 1a0a

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