1a0p

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[[Image:1a0p.jpg|left|200px]]
 
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==SITE-SPECIFIC RECOMBINASE, XERD==
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The line below this paragraph, containing "STRUCTURE_1a0p", creates the "Structure Box" on the page.
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<StructureSection load='1a0p' size='340' side='right'caption='[[1a0p]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1a0p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A0P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A0P FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a0p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a0p OCA], [https://pdbe.org/1a0p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a0p RCSB], [https://www.ebi.ac.uk/pdbsum/1a0p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a0p ProSAT]</span></td></tr>
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{{STRUCTURE_1a0p| PDB=1a0p | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/XERD_ECOLI XERD_ECOLI] Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. Binds cooperatively to specific DNA consensus sequences that are separated from XerC binding sites by a short central region, forming the heterotetrameric XerC-XerD complex that recombines DNA substrates. The complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell division. It also contributes to the segregational stability of plasmids at ColE1 xer (or cer) and pSC101 (or psi) sites. In the complex XerD specifically exchanges the bottom DNA strands (By similarity).<ref>PMID:7744017</ref> <ref>PMID:9268326</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a0/1a0p_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a0p ConSurf].
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<div style="clear:both"></div>
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'''SITE-SPECIFIC RECOMBINASE, XERD'''
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==See Also==
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*[[Resolvase 3D structures|Resolvase 3D structures]]
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== References ==
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==Overview==
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<references/>
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The structure of the site-specific recombinase, XerD, that functions in circular chromosome separation, has been solved at 2.5 A resolution and reveals that the protein comprises two domains. The C-terminal domain contains two conserved sequence motifs that are located in similar positions in the structures of XerD, lambda and HP1 integrases. However, the extreme C-terminal regions of the three proteins, containing the active site tyrosine, are very different. In XerD, the arrangement of active site residues supports a cis cleavage mechanism. Biochemical evidence for DNA bending is encompassed in a model that accommodates extensive biochemical and genetic data, and in which the DNA is wrapped around an alpha-helix in a manner similar to that observed for CAP complexed with DNA.
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__TOC__
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</StructureSection>
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==About this Structure==
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1A0P is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A0P OCA].
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==Reference==
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Crystal structure of the site-specific recombinase, XerD., Subramanya HS, Arciszewska LK, Baker RA, Bird LE, Sherratt DJ, Wigley DB, EMBO J. 1997 Sep 1;16(17):5178-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9311978 9311978]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Arciszewska, L K.]]
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[[Category: Arciszewska LK]]
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[[Category: Baker, R A.]]
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[[Category: Baker RA]]
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[[Category: Bird, L E.]]
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[[Category: Bird LE]]
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[[Category: Sherratt, D J.]]
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[[Category: Sherratt DJ]]
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[[Category: Subramanya, H S.]]
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[[Category: Subramanya HS]]
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[[Category: Wigley, D B.]]
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[[Category: Wigley DB]]
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[[Category: Dna binding]]
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[[Category: Dna recombination]]
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[[Category: Recombinase]]
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[[Category: Xerd]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:38:20 2008''
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Current revision

SITE-SPECIFIC RECOMBINASE, XERD

PDB ID 1a0p

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