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| | ==H3-H3-H3 tri-nucleosome with the 22 base-pair linker DNA== | | ==H3-H3-H3 tri-nucleosome with the 22 base-pair linker DNA== |
| - | <StructureSection load='6l4a' size='340' side='right'caption='[[6l4a]], [[Resolution|resolution]] 12.30Å' scene=''> | + | <SX load='6l4a' size='340' side='right' viewer='molstar' caption='[[6l4a]], [[Resolution|resolution]] 12.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6l4a]] is a 26 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L4A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6L4A FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6l4a]] is a 26 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L4A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L4A FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6l4a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l4a OCA], [http://pdbe.org/6l4a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l4a RCSB], [http://www.ebi.ac.uk/pdbsum/6l4a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l4a ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 12.3Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l4a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l4a OCA], [https://pdbe.org/6l4a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l4a RCSB], [https://www.ebi.ac.uk/pdbsum/6l4a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l4a ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/H2B1J_HUMAN H2B1J_HUMAN]] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.<ref>PMID:11859126</ref> <ref>PMID:12860195</ref> <ref>PMID:15019208</ref> Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.<ref>PMID:11859126</ref> <ref>PMID:12860195</ref> <ref>PMID:15019208</ref> | + | [https://www.uniprot.org/uniprot/H31_HUMAN H31_HUMAN] |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | The histone H3 variant CENP-A is a crucial epigenetic marker for centromere specification. CENP-A forms a characteristic nucleosome and dictates the higher-order configuration of centromeric chromatin. However, little is known about how the CENP-A nucleosome affects the architecture of centromeric chromatin. In this study, we reconstituted tri-nucleosomes mimicking a centromeric nucleosome arrangement containing the CENP-A nucleosome, and determined their 3D structures by cryoelectron microscopy. The H3-CENP-A-H3 tri-nucleosomes adopt an untwisted architecture, with an outward-facing linker DNA path between nucleosomes. This is distinct from the H3-H3-H3 tri-nucleosome architecture, with an inward-facing DNA path. Intriguingly, the untwisted architecture may allow the CENP-A nucleosome to be exposed to the solvent in the condensed chromatin model. These results provide a structural basis for understanding the 3D configuration of CENP-A-containing chromatin, and may explain how centromeric proteins can specifically target the CENP-A nucleosomes buried in robust amounts of H3 nucleosomes in centromeres.
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| - | Cryo-EM Structures of Centromeric Tri-nucleosomes Containing a Central CENP-A Nucleosome.,Takizawa Y, Ho CH, Tachiwana H, Matsunami H, Kobayashi W, Suzuki M, Arimura Y, Hori T, Fukagawa T, Ohi MD, Wolf M, Kurumizaka H Structure. 2019 Nov 7. pii: S0969-2126(19)30357-0. doi:, 10.1016/j.str.2019.10.016. PMID:31711756<ref>PMID:31711756</ref>
| + | ==See Also== |
| - | | + | *[[Histone 3D structures|Histone 3D structures]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6l4a" style="background-color:#fffaf0;"></div>
| + | |
| - | == References == | + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| - | </StructureSection> | + | </SX> |
| | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ho, C H]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Kurumizaka, H]] | + | [[Category: Ho C-H]] |
| - | [[Category: Matsunami, H]] | + | [[Category: Kurumizaka H]] |
| - | [[Category: Ohi, M]] | + | [[Category: Matsunami H]] |
| - | [[Category: Tachiwana, H]] | + | [[Category: Ohi M]] |
| - | [[Category: Takizawa, Y]] | + | [[Category: Tachiwana H]] |
| - | [[Category: Wolf, M]] | + | [[Category: Takizawa Y]] |
| - | [[Category: Centromere]]
| + | [[Category: Wolf M]] |
| - | [[Category: Chromatin]]
| + | |
| - | [[Category: Nuclear protein]]
| + | |
| - | [[Category: Nucleosome]]
| + | |
