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| | <StructureSection load='1rgv' size='340' side='right'caption='[[1rgv]], [[Resolution|resolution]] 2.90Å' scene=''> | | <StructureSection load='1rgv' size='340' side='right'caption='[[1rgv]], [[Resolution|resolution]] 2.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1rgv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thauera_aromatica Thauera aromatica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RGV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RGV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1rgv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thauera_aromatica_K172 Thauera aromatica K172]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RGV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RGV FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rgv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rgv OCA], [http://pdbe.org/1rgv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rgv RCSB], [http://www.ebi.ac.uk/pdbsum/1rgv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1rgv ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rgv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rgv OCA], [https://pdbe.org/1rgv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rgv RCSB], [https://www.ebi.ac.uk/pdbsum/1rgv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rgv ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/O88151_THAAR O88151_THAAR] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Thauera aromatica]] | + | [[Category: Thauera aromatica K172]] |
| - | [[Category: Boll, M]] | + | [[Category: Boll M]] |
| - | [[Category: Ermler, U]] | + | [[Category: Ermler U]] |
| - | [[Category: Unciuleac, M]] | + | [[Category: Unciuleac M]] |
| - | [[Category: Warkentin, E]] | + | [[Category: Warkentin E]] |
| - | [[Category: Electron transport]]
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| Structural highlights
Function
O88151_THAAR
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
4-Hydroxybenzoyl-CoA reductase (4-HBCR) is a central enzyme in the metabolism of phenolic compounds in anaerobic bacteria. The enzyme catalyzes the reductive removal of the phenolic hydroxyl group from 4-hydroxybenzoyl-CoA, yielding benzoyl-CoA and water. 4-HBCR belongs to the xanthine oxidase (XO) family of molybdenum enzymes which occur as heterodimers, (alphabetagamma)(2). 4-HBCR contains two molybdopterins, four [2Fe-2S] and two [4Fe-4S] clusters and two FADs. A low-potential Allochromatium vinosum-type ferredoxin containing two [4Fe-4S] clusters serves as an in vivo electron donor for 4-HBCR. In this work, the oxygen-sensitive proteins 4-HBCR and the ferredoxin (TaFd) from Thauera aromatica were crystallized under anaerobic conditions. 4-HBCR crystallized with PEG 4000 and MPD as precipitant diffracted to about 1.6 A resolution and the crystals were highly suitable for X-ray structure analysis. Crystals of TaFd were obtained with (NH(4))(3)PO(4) as precipitant and revealed a solvent content of 77%, which is remarkably high for a small soluble protein. The structure of TaFd was solved at 2.9 A resolution by the molecular-replacement method using the highly related structure of the ferredoxin (CvFd) from A. vinosum as a model. Structural changes between the two ferredoxins around the [4Fe-4S] cluster can be correlated with their different redox potentials.
Crystallization of 4-hydroxybenzoyl-CoA reductase and the structure of its electron donor ferredoxin.,Unciuleac M, Boll M, Warkentin E, Ermler U Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):388-91. Epub 2004, Jan 23. PMID:14747735[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Unciuleac M, Boll M, Warkentin E, Ermler U. Crystallization of 4-hydroxybenzoyl-CoA reductase and the structure of its electron donor ferredoxin. Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):388-91. Epub 2004, Jan 23. PMID:14747735 doi:10.1107/S0907444903028506
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