6t7o

From Proteopedia

(Difference between revisions)

Current revision

X-ray structure of the C-terminal domain of S. aureus Hibernating Promoting Factor (CTD-SaHPF)

Structural highlights

6t7o is a 2 chain structure with sequence from Staphylococcus aureus subsp. aureus NCTC 8325. This structure supersedes the now removed PDB entry 5oox. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.6000363Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

HPF_STAA8 Required for dimerization of active 70S ribosomes into 100S ribosomes; when added to monomeric 70S ribosomes stimulates formation of 100S dimeric ribosomes. Unlike E.coli, 100S ribosomes are present during exponential growth, peak during early stationary phase and then decrease (shown for strain NBRC 3060).[HAMAP-Rule:MF_00839]^[1]

Publication Abstract from PubMed

Staphylococcus aureus hibernation promoting factor (SaHPF) is responsible for the formation of 100S ribosome dimers, which in turn help this pathogen to reduce energy spent under unfavorable conditions. Ribosome dimer formation strongly depends on the dimerization of the C-terminal domain of SaHPF (CTD(SaHPF)). In this study, we solved the crystal structure of CTD(SaHPF) at 1.6A resolution and obtained a precise arrangement of the dimer interface. Residues Phe(160), Val(162), Thr(171), Ile(173), Tyr(175), Ile(185) andThr(187) in the dimer interface of SaHPF protein were mutated and the effects were analyzed for the formation of 100S disomes of ribosomes isolated from S. aureus. It was shown that substitution of any of single residues Phe(160), Val(162), Ile(173), Tyr(175) and Ile(185) in the SaHPF homodimer interface abolished the ribosome dimerization in vitro.

Dimerization of long hibernation promoting factor from Staphylococcus aureus: Structural analysis and biochemical characterization.,Usachev KS, Fatkhullin BF, Klochkova EA, Miftakhov AK, Golubev AA, Bikmullin AG, Nurullina LI, Garaeva NS, Islamov DR, Gabdulkhakov AG, Lekontseva NV, Tishchenko SV, Balobanov VA, Khusainov IS, Yusupov MM, Validov SZ J Struct Biol. 2019 Oct 25:107408. doi: 10.1016/j.jsb.2019.107408. PMID:31669310^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ueta M, Wada C, Wada A. Formation of 100S ribosomes in Staphylococcus aureus by the hibernation promoting factor homolog SaHPF. Genes Cells. 2010 Jan;15(1):43-58. doi: 10.1111/j.1365-2443.2009.01364.x. Epub, 2009 Dec 15. PMID:20015224 doi:http://dx.doi.org/10.1111/j.1365-2443.2009.01364.x
↑ Usachev KS, Fatkhullin BF, Klochkova EA, Miftakhov AK, Golubev AA, Bikmullin AG, Nurullina LI, Garaeva NS, Islamov DR, Gabdulkhakov AG, Lekontseva NV, Tishchenko SV, Balobanov VA, Khusainov IS, Yusupov MM, Validov SZ. Dimerization of long hibernation promoting factor from Staphylococcus aureus: Structural analysis and biochemical characterization. J Struct Biol. 2019 Oct 25:107408. doi: 10.1016/j.jsb.2019.107408. PMID:31669310 doi:http://dx.doi.org/10.1016/j.jsb.2019.107408

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6t7o"

@@ Line 3: / Line 3: @@
 <StructureSection load='6t7o' size='340' side='right'caption='[[6t7o]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6t7o]] is a 2 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5oox 5oox]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T7O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6T7O FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6t7o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_NCTC_8325 Staphylococcus aureus subsp. aureus NCTC 8325]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5oox 5oox]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6T7O FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6t7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t7o OCA], [http://pdbe.org/6t7o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6t7o RCSB], [http://www.ebi.ac.uk/pdbsum/6t7o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6t7o ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6000363&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6t7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t7o OCA], [https://pdbe.org/6t7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6t7o RCSB], [https://www.ebi.ac.uk/pdbsum/6t7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6t7o ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/HPF_STAA8 HPF_STAA8]] Required for dimerization of active 70S ribosomes into 100S ribosomes; when added to monomeric 70S ribosomes stimulates formation of 100S dimeric ribosomes. Unlike E.coli, 100S ribosomes are present during exponential growth, peak during early stationary phase and then decrease (shown for strain NBRC 3060).[HAMAP-Rule:MF_00839]<ref>PMID:20015224</ref>
+[https://www.uniprot.org/uniprot/HPF_STAA8 HPF_STAA8] Required for dimerization of active 70S ribosomes into 100S ribosomes; when added to monomeric 70S ribosomes stimulates formation of 100S dimeric ribosomes. Unlike E.coli, 100S ribosomes are present during exponential growth, peak during early stationary phase and then decrease (shown for strain NBRC 3060).[HAMAP-Rule:MF_00839]<ref>PMID:20015224</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 23: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Ayupov, R K]]
+[[Category: Staphylococcus aureus subsp. aureus NCTC 8325]]
-[[Category: Fatkhullin, B F]]
+[[Category: Ayupov RK]]
-[[Category: Gabdulkhakov, A G]]
+[[Category: Fatkhullin BF]]
-[[Category: Khusainov, I S]]
+[[Category: Gabdulkhakov AG]]
-[[Category: Tishchenko, S V]]
+[[Category: Khusainov IS]]
-[[Category: Validov, S Z]]
+[[Category: Tishchenko SV]]
-[[Category: Yusupov, M M]]
+[[Category: Validov SZ]]
-[[Category: Hibernating promoting factor]]
+[[Category: Yusupov MM]]
-[[Category: Hpf]]
-[[Category: Ribosomal protein]]
-[[Category: Ribosome]]
-[[Category: Staphylococcus aureus]]

6t7o

From Proteopedia

Current revision

X-ray structure of the C-terminal domain of S. aureus Hibernating Promoting Factor (CTD-SaHPF)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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