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| | <StructureSection load='4p96' size='340' side='right'caption='[[4p96]], [[Resolution|resolution]] 2.20Å' scene=''> | | <StructureSection load='4p96' size='340' side='right'caption='[[4p96]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4p96]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae_hc-21a1 Vibrio cholerae hc-21a1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P96 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4P96 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4p96]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_HC-21A1 Vibrio cholerae HC-21A1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P96 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4P96 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fadR, VCHC21A1_1913 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=991953 Vibrio cholerae HC-21A1])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p96 OCA], [http://pdbe.org/4p96 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4p96 RCSB], [http://www.ebi.ac.uk/pdbsum/4p96 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4p96 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4p96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p96 OCA], [https://pdbe.org/4p96 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4p96 RCSB], [https://www.ebi.ac.uk/pdbsum/4p96 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4p96 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/G6ZTG9_VIBCL G6ZTG9_VIBCL]] Multifunctional regulator of fatty acid metabolism.[HAMAP-Rule:MF_00696] | + | [https://www.uniprot.org/uniprot/A0A0H2UKZ1_VIBCL A0A0H2UKZ1_VIBCL] Multifunctional regulator of fatty acid metabolism.[HAMAP-Rule:MF_00696] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Vibrio cholerae hc-21a1]] | + | [[Category: Vibrio cholerae HC-21A1]] |
| - | [[Category: Kull, F J]] | + | [[Category: Kull FJ]] |
| - | [[Category: Shi, W]] | + | [[Category: Shi W]] |
| - | [[Category: Transcription]]
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| - | [[Category: Transcriptional regulation]]
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| Structural highlights
Function
A0A0H2UKZ1_VIBCL Multifunctional regulator of fatty acid metabolism.[HAMAP-Rule:MF_00696]
Publication Abstract from PubMed
FadR is a master regulator of fatty acid metabolism and influences virulence in certain members of Vibrionaceae. Among FadR homologues of the GntR family, the Vibrionaceae protein is unusual in that it contains a C-terminal 40-residue insertion. Here we report the structure of Vibrio cholerae FadR (VcFadR) alone, bound to DNA, and in the presence of a ligand, oleoyl-CoA. Whereas Escherichia coli FadR (EcFadR) contains only one acyl-CoA-binding site in each monomer, crystallographic and calorimetric data indicate that VcFadR has two. One of the binding sites resembles that of EcFadR, whereas the other, comprised residues from the insertion, has not previously been observed. Upon ligand binding, VcFadR undergoes a dramatic conformational change that would more fully disrupt DNA binding than EcFadR. These findings suggest that the ability to bind and respond to an additional ligand allows FadR from Vibrionaceae to function as a more efficient regulator.
The 40-residue insertion in Vibrio cholerae FadR facilitates binding of an additional fatty acyl-CoA ligand.,Shi W, Kovacikova G, Lin W, Taylor RK, Skorupski K, Kull FJ Nat Commun. 2015 Jan 21;6:6032. doi: 10.1038/ncomms7032. PMID:25607896[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Shi W, Kovacikova G, Lin W, Taylor RK, Skorupski K, Kull FJ. The 40-residue insertion in Vibrio cholerae FadR facilitates binding of an additional fatty acyl-CoA ligand. Nat Commun. 2015 Jan 21;6:6032. doi: 10.1038/ncomms7032. PMID:25607896 doi:http://dx.doi.org/10.1038/ncomms7032
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