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| | <StructureSection load='6anw' size='340' side='right'caption='[[6anw]], [[Resolution|resolution]] 2.49Å' scene=''> | | <StructureSection load='6anw' size='340' side='right'caption='[[6anw]], [[Resolution|resolution]] 2.49Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6anw]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Cctcc_m_209017 Cctcc m 209017]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ANW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ANW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6anw]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_xiamenensis Shewanella xiamenensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ANW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ANW FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SXM_1276 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=332186 CCTCC M 209017])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.486Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6anw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6anw OCA], [http://pdbe.org/6anw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6anw RCSB], [http://www.ebi.ac.uk/pdbsum/6anw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6anw ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6anw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6anw OCA], [https://pdbe.org/6anw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6anw RCSB], [https://www.ebi.ac.uk/pdbsum/6anw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6anw ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/A0A073KP86_9GAMM A0A073KP86_9GAMM] |
| - | Prokaryotic cells possess CRISPR-mediated adaptive immune systems that protect them from foreign genetic elements, such as invading viruses. A central element of this immune system is an RNA-guided surveillance complex capable of targeting non-self DNA or RNA for degradation in a sequence- and site-specific manner analogous to RNA interference. Although the complexes display considerable diversity in their composition and architecture, many basic mechanisms underlying target recognition and cleavage are highly conserved. Using cryoelectron microscopy (cryo-EM), we show that the binding of target double-stranded DNA (dsDNA) to a type I-F CRISPR system yersinia (Csy) surveillance complex leads to large quaternary and tertiary structural changes in the complex that are likely necessary in the pathway leading to target dsDNA degradation by a trans-acting helicase-nuclease. Comparison of the structure of the surveillance complex before and after dsDNA binding, or in complex with three virally encoded anti-CRISPR suppressors that inhibit dsDNA binding, reveals mechanistic details underlying target recognition and inhibition.
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| - | | + | |
| - | Cryo-EM Structures Reveal Mechanism and Inhibition of DNA Targeting by a CRISPR-Cas Surveillance Complex.,Guo TW, Bartesaghi A, Yang H, Falconieri V, Rao P, Merk A, Eng ET, Raczkowski AM, Fox T, Earl LA, Patel DJ, Subramaniam S Cell. 2017 Oct 5;171(2):414-426.e12. doi: 10.1016/j.cell.2017.09.006. PMID:28985564<ref>PMID:28985564</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6anw" style="background-color:#fffaf0;"></div>
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| | | | |
| | ==See Also== | | ==See Also== |
| | *[[CRISPR subtype I-F|CRISPR subtype I-F]] | | *[[CRISPR subtype I-F|CRISPR subtype I-F]] |
| - | == References == | |
| - | <references/> | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cctcc m 209017]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Patel, D J]] | + | [[Category: Shewanella xiamenensis]] |
| - | [[Category: Yang, H]] | + | [[Category: Patel DJ]] |
| - | [[Category: Immune system]] | + | [[Category: Yang H]] |
| - | [[Category: Type i-f crispr-cas system: csy cascade: structure: anti-crispr protein: inhibition of csy complex: genome editing tool]]
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