|
|
| (One intermediate revision not shown.) |
| Line 3: |
Line 3: |
| | <StructureSection load='2bsf' size='340' side='right'caption='[[2bsf]], [[Resolution|resolution]] 2.10Å' scene=''> | | <StructureSection load='2bsf' size='340' side='right'caption='[[2bsf]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2bsf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Avian_orthoreovirus Avian orthoreovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BSF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BSF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2bsf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Avian_orthoreovirus Avian orthoreovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BSF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BSF FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2bt7|2bt7]], [[2bt8|2bt8]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bsf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bsf OCA], [http://pdbe.org/2bsf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bsf RCSB], [http://www.ebi.ac.uk/pdbsum/2bsf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2bsf ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bsf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bsf OCA], [https://pdbe.org/2bsf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bsf RCSB], [https://www.ebi.ac.uk/pdbsum/2bsf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bsf ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/SIGC_ARVS1 SIGC_ARVS1] Structural protein responsible for cell attachment. Induces cell apoptosis.<ref>PMID:15033566</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| Line 27: |
Line 29: |
| | </div> | | </div> |
| | <div class="pdbe-citations 2bsf" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 2bsf" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| Line 33: |
Line 38: |
| | [[Category: Avian orthoreovirus]] | | [[Category: Avian orthoreovirus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Calvo, P Guardado]]
| + | [[Category: Fox GC]] |
| - | [[Category: Fox, G C]] | + | [[Category: Guardado Calvo P]] |
| - | [[Category: Llamas-Saiz, A L]] | + | [[Category: Hermo Parrado XL]] |
| - | [[Category: Parrado, X L.Hermo]] | + | [[Category: Llamas-Saiz AL]] |
| - | [[Category: Raaij, M J.van]] | + | [[Category: Van Raaij MJ]] |
| - | [[Category: Beta-barrel]]
| + | |
| - | [[Category: Orthoreovirus]] | + | |
| - | [[Category: Triple beta-spiral]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| Structural highlights
Function
SIGC_ARVS1 Structural protein responsible for cell attachment. Induces cell apoptosis.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Avian reovirus fibre, a homo-trimer of the sigmaC protein, is responsible for primary host cell attachment. The protein expressed in bacteria forms elongated fibres comprised of a carboxy-terminal globular head domain and a slender shaft, and partial proteolysis yielded a carboxy-terminal protease-stable domain that was amenable to crystallisation. Here, we show that this fragment retains receptor-binding capability and report its structure, solved using two-wavelength anomalous diffraction and refined using data collected from three different crystal forms at 2.1 angstroms, 2.35 angstroms and 3.0 angstroms resolution. The carboxy-terminal globular domain has a beta-barrel fold with the same overall topology as the mammalian reovirus fibre (sigma1). However, the monomers of the sigmaC trimer show a more splayed-out arrangement than in the sigma1 structure. Also resolved are two triple beta-spiral repeats of the shaft or stalk domain. The presence in the sequence of heptad repeats amino-terminal to these triple beta-spiral repeats suggests that the unresolved portion of the shaft domain contains a triple alpha-helical coiled-coil structure. Implications for the function and stability of the sigmaC protein are discussed.
Structure of the carboxy-terminal receptor-binding domain of avian reovirus fibre sigmaC.,Guardado Calvo P, Fox GC, Hermo Parrado XL, Llamas-Saiz AL, Costas C, Martinez-Costas J, Benavente J, van Raaij MJ J Mol Biol. 2005 Nov 18;354(1):137-49. Epub 2005 Sep 30. PMID:16236316[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Shih WL, Hsu HW, Liao MH, Lee LH, Liu HJ. Avian reovirus sigmaC protein induces apoptosis in cultured cells. Virology. 2004 Mar 30;321(1):65-74. PMID:15033566 doi:10.1016/j.virol.2003.12.004
- ↑ Guardado Calvo P, Fox GC, Hermo Parrado XL, Llamas-Saiz AL, Costas C, Martinez-Costas J, Benavente J, van Raaij MJ. Structure of the carboxy-terminal receptor-binding domain of avian reovirus fibre sigmaC. J Mol Biol. 2005 Nov 18;354(1):137-49. Epub 2005 Sep 30. PMID:16236316 doi:10.1016/j.jmb.2005.09.034
|
