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2ca7

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Current revision

Conkunitzin-S1 Is The First Member Of A New Kunitz-Type Neurotoxin Family- Structural and Functional Characterization

Structural highlights

2ca7 is a 1 chain structure with sequence from Conus striatus. This structure supersedes the now removed PDB entry 1yl2. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

VKTS1_CONST Blocks specifically voltage-activated potassium channels (Kv) of the Shaker family (IC(50)=1.33 nM).^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Conkunitzin-S1 (Conk-S1) is a 60-residue neurotoxin from the venom of the cone snail Conus striatus that interacts with voltage-gated potassium channels. Conk-S1 shares sequence homology with Kunitz-type proteins but contains only two out of the three highly conserved cysteine bridges, which are typically found in these small, basic protein modules. In this study the three-dimensional structure of Conk-S1 has been solved by multidimensional NMR spectroscopy. The solution structure of recombinant Conk-S1 shows that a Kunitz fold is present, even though one of the highly conserved disulfide cross-links is missing. Introduction of a third, homologous disulfide bond into Conk-S1 results in a functional toxin with similar affinity for Shaker potassium channels. The affinity of Conk-S1 can be enhanced by a pore mutation within the Shaker channel pore indicating an interaction of Conk-S1 with the vestibule of potassium channels.

Conkunitzin-S1 is the first member of a new Kunitz-type neurotoxin family. Structural and functional characterization.,Bayrhuber M, Vijayan V, Ferber M, Graf R, Korukottu J, Imperial J, Garrett JE, Olivera BM, Terlau H, Zweckstetter M, Becker S J Biol Chem. 2005 Jun 24;280(25):23766-70. Epub 2005 Apr 15. PMID:15833744^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bayrhuber M, Vijayan V, Ferber M, Graf R, Korukottu J, Imperial J, Garrett JE, Olivera BM, Terlau H, Zweckstetter M, Becker S. Conkunitzin-S1 is the first member of a new Kunitz-type neurotoxin family. Structural and functional characterization. J Biol Chem. 2005 Jun 24;280(25):23766-70. Epub 2005 Apr 15. PMID:15833744 doi:10.1074/jbc.C500064200
↑ Bayrhuber M, Vijayan V, Ferber M, Graf R, Korukottu J, Imperial J, Garrett JE, Olivera BM, Terlau H, Zweckstetter M, Becker S. Conkunitzin-S1 is the first member of a new Kunitz-type neurotoxin family. Structural and functional characterization. J Biol Chem. 2005 Jun 24;280(25):23766-70. Epub 2005 Apr 15. PMID:15833744 doi:10.1074/jbc.C500064200

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ca7"

@@ Line 1: / Line 1: @@
 ==Conkunitzin-S1 Is The First Member Of A New Kunitz-Type Neurotoxin Family- Structural and Functional Characterization==
-<StructureSection load='2ca7' size='340' side='right'caption='[[2ca7]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2ca7' size='340' side='right'caption='[[2ca7]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2ca7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Const Const]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1yl2 1yl2]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CA7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CA7 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2ca7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Conus_striatus Conus striatus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1yl2 1yl2]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CA7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CA7 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ca7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ca7 OCA], [http://pdbe.org/2ca7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ca7 RCSB], [http://www.ebi.ac.uk/pdbsum/2ca7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ca7 ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ca7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ca7 OCA], [https://pdbe.org/2ca7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ca7 RCSB], [https://www.ebi.ac.uk/pdbsum/2ca7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ca7 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/VKTS1_CONST VKTS1_CONST] Blocks specifically voltage-activated potassium channels (Kv) of the Shaker family (IC(50)=1.33 nM).<ref>PMID:15833744</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 29: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Const]]
+[[Category: Conus striatus]]
 [[Category: Large Structures]]
-[[Category: Bayrhuber, M]]
+[[Category: Bayrhuber M]]
-[[Category: Becker, S]]
+[[Category: Becker S]]
-[[Category: Ferber, M]]
+[[Category: Ferber M]]
-[[Category: Garrett, J E]]
+[[Category: Garrett JE]]
-[[Category: Graf, R]]
+[[Category: Graf R]]
-[[Category: Imperial, J]]
+[[Category: Imperial J]]
-[[Category: Korukottu, J]]
+[[Category: Korukottu J]]
-[[Category: Olivera, B M]]
+[[Category: Olivera BM]]
-[[Category: Terlau, H]]
+[[Category: Terlau H]]
-[[Category: Vijayan, V]]
+[[Category: Vijayan V]]
-[[Category: Zweckstetter, M]]
+[[Category: Zweckstetter M]]
-[[Category: Conkunitzin]]
-[[Category: Kunitz-domain]]
-[[Category: Kunitz-type fold]]
-[[Category: Neurotoxin]]
-[[Category: Potassium channel inhibitor]]
-[[Category: Toxin]]

2ca7

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Current revision

Conkunitzin-S1 Is The First Member Of A New Kunitz-Type Neurotoxin Family- Structural and Functional Characterization

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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