6l2d
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of a cupin protein (tm1459) in copper (Cu) substituted form== | |
| + | <StructureSection load='6l2d' size='340' side='right'caption='[[6l2d]], [[Resolution|resolution]] 1.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6l2d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L2D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L2D FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.198Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l2d OCA], [https://pdbe.org/6l2d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l2d RCSB], [https://www.ebi.ac.uk/pdbsum/6l2d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l2d ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q9X1H0_THEMA Q9X1H0_THEMA] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cupin superfamily proteins (TM1459) work as a macromolecular ligand framework with a double-stranded beta-barrel structure ligating to a Cu ion through histidine side chains. Variegating the first coordination sphere of TM1459 revealed that H52A and H54A/H58A mutants effectively catalyzed the diastereo- and enantioselective Michael addition reaction of nitroalkanes to an alpha,beta-unsaturated ketone. Moreover, calculated substrate docking signified C106N and F104W single-point mutations, which inverted the diastereoselectivity of H52A and further improved the stereoselectivity of H54A/H58A, respectively. | ||
| - | + | Cupin Variants as a Macromolecular Ligand Library for Stereoselective Michael Addition of Nitroalkanes.,Fujieda N, Ichihashi H, Yuasa M, Nishikawa Y, Kurisu G, Itoh S Angew Chem Int Ed Engl. 2020 Feb 19. doi: 10.1002/anie.202000129. PMID:32073197<ref>PMID:32073197</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6l2d" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: Itoh | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| + | [[Category: Large Structures]] | ||
| + | [[Category: Thermotoga maritima MSB8]] | ||
| + | [[Category: Fujieda N]] | ||
| + | [[Category: Ichihashi H]] | ||
| + | [[Category: Itoh S]] | ||
| + | [[Category: Kurisu G]] | ||
| + | [[Category: Nishikawa Y]] | ||
Current revision
Crystal structure of a cupin protein (tm1459) in copper (Cu) substituted form
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