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6tea
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Tail of native GTA particle computed with helical refinement, C6 symmetry== | |
| + | <StructureSection load='6tea' size='340' side='right'caption='[[6tea]], [[Resolution|resolution]] 3.89Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6tea]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodobacter_capsulatus Rhodobacter capsulatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TEA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TEA FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.89Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tea OCA], [https://pdbe.org/6tea PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tea RCSB], [https://www.ebi.ac.uk/pdbsum/6tea PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tea ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/D5ATZ7_RHOCB D5ATZ7_RHOCB] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Alphaproteobacteria, which are the most abundant microorganisms of temperate oceans, produce phage-like particles called gene transfer agents (GTAs) that mediate lateral gene exchange. However, the mechanism by which GTAs deliver DNA into cells is unknown. Here we present the structure of the GTA of Rhodobacter capsulatus (RcGTA) and describe the conformational changes required for its DNA ejection. The structure of RcGTA resembles that of a tailed phage, but it has an oblate head shortened in the direction of the tail axis, which limits its packaging capacity to less than 4,500 base pairs of linear double-stranded DNA. The tail channel of RcGTA contains a trimer of proteins that possess features of both tape measure proteins of long-tailed phages from the family Siphoviridae and tail needle proteins of short-tailed phages from the family Podoviridae. The opening of a constriction within the RcGTA baseplate enables the ejection of DNA into bacterial periplasm. | ||
| - | + | Structure and mechanism of DNA delivery of a gene transfer agent.,Bardy P, Fuzik T, Hrebik D, Pantucek R, Thomas Beatty J, Plevka P Nat Commun. 2020 Jun 15;11(1):3034. doi: 10.1038/s41467-020-16669-9. PMID:32541663<ref>PMID:32541663</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6tea" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Rhodobacter capsulatus]] | ||
| + | [[Category: Bardy P]] | ||
| + | [[Category: Beatty JT]] | ||
| + | [[Category: Fuzik T]] | ||
| + | [[Category: Hrebik D]] | ||
| + | [[Category: Pantucek R]] | ||
| + | [[Category: Plevka P]] | ||
Current revision
Tail of native GTA particle computed with helical refinement, C6 symmetry
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