5t4n

From Proteopedia

(Difference between revisions)

Current revision

Crystal Structure of Human Protocadherin-15 EC3-5 D414A Variant

Structural highlights

5t4n is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.701Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

PCD15_HUMAN Usher syndrome type 1;Autosomal recessive non-syndromic sensorineural deafness type DFNB. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting distinct genetic loci, including the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.

Function

PCD15_HUMAN Calcium-dependent cell-adhesion protein. Essential for maintenance of normal retinal and cochlear function.

Publication Abstract from PubMed

Tip links of the inner ear are protein filaments essential for hearing and balance. Two atypical cadherins, cadherin-23 and protocadherin-15, interact in a Ca(2+)-dependent manner to form tip links. The largely unknown structure and mechanics of these proteins are integral to understanding how tip links pull on ion channels to initiate sensory perception. Protocadherin-15 has 11 extracellular cadherin (EC) repeats. Its EC3-4 linker lacks several of the canonical Ca(2+)-binding residues, and contains an aspartate-to-alanine polymorphism (D414A) under positive selection in East Asian populations. We present structures of protocadherin-15 EC3-5 featuring two Ca(2+)-binding linker regions: canonical EC4-5 linker binding three Ca(2+) ions, and non-canonical EC3-4 linker binding only two Ca(2+) ions. Our structures and biochemical assays reveal little difference between the D414 and D414A variants. Simulations predict that the partial Ca(2+)-free EC3-4 linker exhibits increased flexural flexibility without compromised mechanical strength, providing insight into the dynamics of tip links and other atypical cadherins.

A Partial Calcium-Free Linker Confers Flexibility to Inner-Ear Protocadherin-15.,Powers RE, Gaudet R, Sotomayor M Structure. 2017 Mar 7;25(3):482-495. doi: 10.1016/j.str.2017.01.014. Epub 2017, Feb 23. PMID:28238533^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Powers RE, Gaudet R, Sotomayor M. A Partial Calcium-Free Linker Confers Flexibility to Inner-Ear Protocadherin-15. Structure. 2017 Mar 7;25(3):482-495. doi: 10.1016/j.str.2017.01.014. Epub 2017, Feb 23. PMID:28238533 doi:http://dx.doi.org/10.1016/j.str.2017.01.014

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5t4n"

Categories: Homo sapiens | Large Structures | Gaudet R | Powers RE | Sotomayor M

@@ Line 3: / Line 3: @@
 <StructureSection load='5t4n' size='340' side='right'caption='[[5t4n]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5t4n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T4N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5T4N FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5t4n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T4N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5T4N FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.701&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5t4m|5t4m]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PCDH15, USH1F ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5t4n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t4n OCA], [https://pdbe.org/5t4n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5t4n RCSB], [https://www.ebi.ac.uk/pdbsum/5t4n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5t4n ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5t4n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t4n OCA], [http://pdbe.org/5t4n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t4n RCSB], [http://www.ebi.ac.uk/pdbsum/5t4n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t4n ProSAT]</span></td></tr>
 </table>
 == Disease ==
-[[http://www.uniprot.org/uniprot/PCD15_HUMAN PCD15_HUMAN]] Usher syndrome type 1;Autosomal recessive non-syndromic sensorineural deafness type DFNB. The disease is caused by mutations affecting the gene represented in this entry.  The disease is caused by mutations affecting distinct genetic loci, including the gene represented in this entry.  The disease is caused by mutations affecting the gene represented in this entry.
+[https://www.uniprot.org/uniprot/PCD15_HUMAN PCD15_HUMAN] Usher syndrome type 1;Autosomal recessive non-syndromic sensorineural deafness type DFNB. The disease is caused by mutations affecting the gene represented in this entry.  The disease is caused by mutations affecting distinct genetic loci, including the gene represented in this entry.  The disease is caused by mutations affecting the gene represented in this entry.
 == Function ==
-[[http://www.uniprot.org/uniprot/PCD15_HUMAN PCD15_HUMAN]] Calcium-dependent cell-adhesion protein. Essential for maintenance of normal retinal and cochlear function.
+[https://www.uniprot.org/uniprot/PCD15_HUMAN PCD15_HUMAN] Calcium-dependent cell-adhesion protein. Essential for maintenance of normal retinal and cochlear function.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 21: @@
 </div>
 <div class="pdbe-citations 5t4n" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Cadherin 3D structures|Cadherin 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Gaudet, R]]
+[[Category: Gaudet R]]
-[[Category: Powers, R E]]
+[[Category: Powers RE]]
-[[Category: Sotomayor, M]]
+[[Category: Sotomayor M]]
-[[Category: Cell adhesion]]
-[[Category: Hearing]]
-[[Category: Mechanotransduction]]

5t4n

From Proteopedia

Current revision

Crystal Structure of Human Protocadherin-15 EC3-5 D414A Variant

Structural highlights

Disease

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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