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| | <StructureSection load='6cam' size='340' side='right'caption='[[6cam]], [[Resolution|resolution]] 1.75Å' scene=''> | | <StructureSection load='6cam' size='340' side='right'caption='[[6cam]], [[Resolution|resolution]] 1.75Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6cam]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_25175 Atcc 25175]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CAM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CAM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6cam]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_mutans Streptococcus mutans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CAM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CAM FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5uqz|5uqz]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gbpC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1309 ATCC 25175])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cam OCA], [https://pdbe.org/6cam PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cam RCSB], [https://www.ebi.ac.uk/pdbsum/6cam PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cam ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cam OCA], [http://pdbe.org/6cam PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cam RCSB], [http://www.ebi.ac.uk/pdbsum/6cam PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cam ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8DTF1_STRMU Q8DTF1_STRMU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 25175]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Deivanayagam, C]] | + | [[Category: Streptococcus mutans]] |
| - | [[Category: Mieher, J L]] | + | [[Category: Deivanayagam C]] |
| - | [[Category: Schormann, N]] | + | [[Category: Mieher JL]] |
| - | [[Category: Beta-supersandwich]] | + | [[Category: Schormann N]] |
| - | [[Category: Glucan binding protein]]
| + | |
| - | [[Category: Salivary agglutinin]]
| + | |
| - | [[Category: Sugar binding protein]]
| + | |
| Structural highlights
Function
Q8DTF1_STRMU
Publication Abstract from PubMed
The high-resolution structure of Glucan binding protein C (GbpC) at 1.14 A, a sucrose-dependent virulence factor of the dental caries pathogen Streptococcus mutans, has been determined. GbpC not only shares structural similarities with the V-regions of AgI/II and SspB, but also functional adherence to SAG and its scavenger receptor cysteine rich domains (SRCRs) which is not only a newly identified function for GbpC, but also an additional fail-safe binding mechanism for S. mutans Despite the structural similarities with S. mutans Antigen I/II (AgI/II) and SspB of Streptococcus gordonii, GbpC remains unique among these surface proteins in its propensity to adhere to dextran/glucans. The complex crystal structure of GbpC with dextrose (beta-D glucose, BGC) highlights exclusive structural features that facilitates this interaction with dextran. Targeted deletion mutant studies on GbpC's divergent loop region in the vicinity of a highly-conserved calcium binding site confirm its role in biofilm formation. Finally, we present a model for adherence to dextran. The structure of GbpC highlights how artfully microbes have engineered the lectin-like folds to broaden their functional adherence repertoire.
Glucan Binding Protein C of Streptococcus mutans Mediates both Sucrose-Independent and Sucrose-Dependent Adherence.,Mieher JL, Larson MR, Schormann N, Purushotham S, Wu R, Rajashankar KR, Wu H, Deivanayagam C Infect Immun. 2018 Apr 23. pii: IAI.00146-18. doi: 10.1128/IAI.00146-18. PMID:29685986[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Mieher JL, Larson MR, Schormann N, Purushotham S, Wu R, Rajashankar KR, Wu H, Deivanayagam C. Glucan Binding Protein C of Streptococcus mutans Mediates both Sucrose-Independent and Sucrose-Dependent Adherence. Infect Immun. 2018 Apr 23. pii: IAI.00146-18. doi: 10.1128/IAI.00146-18. PMID:29685986 doi:http://dx.doi.org/10.1128/IAI.00146-18
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